STAT5 A/B pY694/699 ELISA Kit (ab176656)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 5 µg/ml
- Range: 10 µg/ml - 1000 µg/ml
- Sample type: Adherent cells, Cell culture extracts, Suspension cells, Tissue Homogenate
- Detection method: Colorimetric
- Assay type: Semi-quantitative
- Reacts with: Human
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Wash Buffer PT 1 x 15ml 50X Cell Extraction Enhancer Solution 1 x 1ml 5X Cell Extraction Buffer PTR 1 x 12ml Lyophilized STAT5 A/B Control Lysate 1 vial Plate Seal 1 unit SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit STAT5 A/B (pY694/699) Capture Antibody 1 x 3ml STAT5 A/B (pY694/699) Detector Antibody 1 x 3ml Stop Solution 1 x 12ml TMB Substrate 1 x 12ml -
Research areas
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Function
Carries out a dual function: signal transduction and activation of transcription. Binds to the GAS element and activates PRL-induced transcription. -
Sequence similarities
Belongs to the transcription factor STAT family.
Contains 1 SH2 domain. -
Post-translational
modificationsTyrosine phosphorylated in response to IL-2, IL-3, IL-7, IL-15, GM-CSF, growth hormone, prolactin, erythropoietin and thrombopoietin. Tyrosine phosphorylation is required for DNA-binding activity and dimerization. Serine phosphorylation is also required for maximal transcriptional activity. -
Cellular localization
Cytoplasm. Nucleus. Translocated into the nucleus in response to phosphorylation. - Information by UniProt
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Alternative names
- MGF
- Signal transducer and activator of transcription 5A
- Signal Transducer and Activator of Transcription 5B
see all -
Database links
- Entrez Gene: 6777 Human
- Entrez Gene: 6776 Human
- Entrez Gene: 20851 Mouse
- Entrez Gene: 20850 Mouse
- Entrez Gene: 25126 Rat
- Entrez Gene: 24918 Rat
- Omim: 601511 Human
- Omim: 604260 Human
see all
Images
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Other - STAT5 A/B pY694/699 ELISA Kit (ab176656)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Typical cell lysate dilution seriesExample of a typical STAT5 A/B (pY694/699) cell lysate dilution series. Background-subtracted data values (mean +/- SD) are graphed.
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Induction of STAT5 A/B (pY694/699) phosphorylation in A431 cells in response to EGF treatment.A431 cells were cultured in 96-well tissue culture plates and treated (15 min) with a dose-range of EGF before cell lysis. Data from quadruplicate measurements of STAT5 A/B (pY694/699) are plotted and compared against Total STAT5 A/B levels.
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Cell line analysis for Total STAT5 A/BCell line analysis for Total STAT5 A/B from 100 µg/mL preparations of cell extracts. Data from triplicate measurements (mean +/- SD) are plotted and compared to 1X Cell Extraction Buffer PTR (zero).