Anti-CD42b antibody [AK2] - BSA and Azide free (ab252264)
![Anti-CD42b antibody [AK2] - BSA and Azide free (ab252264)](https://www.abcam.com/ps/products/252/ab252264/Images/ab252264-368237-anti-cd42b-antibody-icc-hel-cells.jpg "Anti-CD42b antibody [AK2] - BSA and Azide free (ab252264)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse monoclonal [AK2] to CD42b - BSA and Azide free
- Suitable for: ICC/IF, Flow Cyt
- Reacts with: Mouse, Human
Overview
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Product name
Anti-CD42b antibody [AK2] - BSA and Azide free
See all CD42b primary antibodies -
Description
Mouse monoclonal [AK2] to CD42b - BSA and Azide free -
Host species
Mouse -
Tested applications
Suitable for: ICC/IF, Flow Cytmore details
Unsuitable for: IHC-P,IP or WB -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant fragment. This information is considered to be commercially sensitive.
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Positive control
- ICC/IF: HEL cells and mouse splenocytes. Flow cyt: Human peripheral blood mononuclear cells
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
ab252264 is the carrier-free version of ab61402. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
AK2 -
Isotype
IgG1 -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-CD42b antibody [AK2] - BSA and Azide free (ab252264)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HEL (human Erythroleukemia erythroblast) cells labelling CD42b with ab61402 at 1/50 dilution, followed by ab150113 AlexaFluor®488 Goat anti-Mouse secondary antibody at 1/1000 dilution (Green). Confocal image showing membranous staining in HEL cells. ab179504 Anti-beta IV Tubulin antibody - Microtubule Marker was used to counterstain tubulin at 1/1000 dilution, followed by ab150080 AlexaFluor®594 Goat anti-Rabbit secondary at 1/1000 dilution (Red). The Nuclear counterstain was DAPI (Blue).
The negative controls are as follows:
-ve control 1: ab61402 at 1/100 dilution, followed by ab150080 AlexaFluor®594 Goat anti-Rabbit secondary secondary at 1/1000 dilution.
-ve control 2: ab179504 at 1/200 dilution, followed by ab150113 AlexaFluor®488 Goat anti-Mouse secondary at 1/1000 dilution.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab61402).
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![Flow Cytometry - Anti-CD42b antibody [AK2] - BSA and Azide free (ab252264)](https://www.abcam.com/ps/products/252/ab252264/Images/ab252264-368235-anti-cd42b-antibody-flow-cytometry-human-peripheral-blood-mononuclear-cells.jpg)
Flow Cytometry - Anti-CD42b antibody [AK2] - BSA and Azide free (ab252264)Flow cytometric analysis of Human peripheral blood mononuclear cell (PBMC) cells labelling CD42b with ab61402 at 1/490 (Right) compared with a Mouse IgG isotype control (Left). Goat anti mouse IgG (Alexa Fluor® 488, ab150113) at 1/2000 dilution was used as the secondary antibody.
Cells were stained with mouse IgG (Left) or ab61402 (Right). Then stained with anti-CD41 conjugated to APC. Gated on viable cells.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab61402).
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![Immunocytochemistry/ Immunofluorescence - Anti-CD42b antibody [AK2] - BSA and Azide free (ab252264)](https://www.abcam.com/ps/products/252/ab252264/Images/ab252264-368236-anti-cd42b-antibody-icc-mouse-splenocytes.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-CD42b antibody [AK2] - BSA and Azide free (ab252264)Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized mouse splenocytes cells labelling CD42b with ab61402 at 1/50 dilution, followed by ab150113 AlexaFluor®488 Goat anti-Mouse secondary antibody at 1/1000 dilution (Green). Confocal image showing membranous staining in mouse splenocytes. ab179504 Anti-beta IV Tubulin antibody - Microtubule Marker was used to counterstain tubulin at 1/1000 dilution, followed by ab150080 AlexaFluor®594 Goat anti-Rabbit secondary at 1/1000 dilution (Red). The Nuclear counterstain was DAPI (Blue).
The negative controls are as follows:
-ve control 1: ab61402 at 1/100 dilution, followed by ab150080 AlexaFluor®594 Goat anti-Rabbit secondary secondary at 1/1000 dilution.
-ve control 2: ab179504 at 1/200 dilution, followed by ab150113 AlexaFluor®488 Goat anti-Mouse secondary at 1/1000 dilution.Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150113 AlexaFluor®488 Goat anti-Mouse secondary at 1:1000 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab61402).
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![Anti-CD42b antibody [AK2] - BSA and Azide free (ab252264)](https://www.abcam.com/ps/products/252/ab252264/Images/ab252264-4-benefits-of-recombinant-antibodies.png)
Anti-CD42b antibody [AK2] - BSA and Azide free (ab252264)
