Antibodies, Proteins, Kits and Reagents for Life Science

304 886 ₸ Product size

100 µl 304 886 ₸

Order now and get it on Tuesday March 02, 2021

Anti-CD42b antibody [EPR6995] (ab134087)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR6995] to CD42b
Suitable for: WB, IHC-P
Reacts with: Human

Product name

Anti-CD42b antibody [EPR6995]
See all CD42b primary antibodies
Description

Rabbit monoclonal [EPR6995] to CD42b
Host species

Rabbit
Tested Applications & Species

Application Species
IHC-P
Human

WB
Human

See all applications and species data
Immunogen

Synthetic peptide within Human CD42b aa 1-100. The exact sequence is proprietary.
Positive control
- Human platelet lysate, Human spleen tissue
General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Application	Species
IHC-P	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (K_D)

K_D = 4.70 x 10 ^-11 M
Learn more about K_D
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.21% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR6995
Isotype

IgG
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD42b antibody [EPR6995] (ab134087)

Immunohistochemical analysis of paraffin embedded Human spleen tissue labelling CD42b with ab134087 at a dilution of 1/100.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Western blot - Anti-CD42b antibody [EPR6995] (ab134087)

Anti-CD42b antibody [EPR6995] (ab134087) at 1/10000 dilution + Human platelets at 20 µg

Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

Predicted band size: 69 kDa

Diluting and blocking buffer: 5% NFDM/TBST
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD42b antibody [EPR6995] (ab134087)

ab134087 staining CD42b in human bone marrow tissue by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraffin and antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/250. A goat anti-rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody at 1/500.

Negative control 1: PBS in place of primary antibody.
Western blot - Anti-CD42b antibody [EPR6995] (ab134087)

Anti-CD42b antibody [EPR6995] (ab134087) at 1/10000 dilution + Human platelet lysate at 10 µg

Secondary
HRP labelled Goat anti-rabbit at 1/2000 dilution

Predicted band size: 69 kDa
Observed band size: 143 kDa
why is the actual band size different from the predicted?
OI-RD Scanning - Anti-CD42b antibody [EPR6995] (ab134087)

Equilibrium disassociation constant (K_D)
Learn more about K_D

Click here to learn more about K_D
Anti-CD42b antibody [EPR6995] (ab134087)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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