Anti-CD42b antibody [6H6H10] (ab233794)
Key features and details
- Mouse monoclonal [6H6H10] to CD42b
- Suitable for: WB, Flow Cyt
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-CD42b antibody [6H6H10]
See all CD42b primary antibodies -
Description
Mouse monoclonal [6H6H10] to CD42b -
Host species
Mouse -
Tested applications
Suitable for: WB, Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human CD42b aa 1-200 (extracellular). (Expressed in E.coli).
Database link: P07359 -
Positive control
- WB: HL-60, MOLT-4 and Ramos celll lysates; CD42b (aa 17-183)-hIgGFc transfected HEK-293 cell lysate; Human CD42b (aa 17-183) recombinant protein. Flow Cyt: HL-60 and Jurkat cells.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purified from tissue culture supernatant. -
Clonality
Monoclonal -
Clone number
6H6H10 -
Isotype
IgG1 -
Research areas
Images
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Anti-CD42b antibody [6H6H10] (ab233794) at 1/500 dilution + Human CD42b (aa 17-183) recombinant protein
Predicted band size: 72 kDaExpected MW is 44.2 kDa.
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All lanes : Anti-CD42b antibody [6H6H10] (ab233794) at 1/500 dilution
Lane 1 : HEK-293 (human epithelial cell line from embryonic kidney) cell lysate
Lane 2 : CD42b (aa 17-183)-hIgGFc transfected HEK-293 cell lysate
Predicted band size: 72 kDa
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All lanes : Anti-CD42b antibody [6H6H10] (ab233794) at 1/500 dilution
Lane 1 : HL-60 (human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 2 : MOLT-4 (human lymphoblastic leukemia cell line) cell lysate
Lane 3 : Ramos (human Burkitt's lymphoma cell line) cell lysate
Predicted band size: 72 kDa
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Flow cytometric analysis of HL-60 (human promyelocytic leukemia cell line) cells labeling CD42b with ab233794 at 1/200 dilution (green) compared with a negative control (red).
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Flow cytometric analysis of Jurkat (human T cell leukemia cell line from peripheral blood) cells labeling CD42b with ab233794 at 1/200 dilution (green) compared with a negative control (red).