Anti-CD42b antibody [SP202] (ab227669)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [SP202] to CD42b
- Suitable for: IHC-P, IHC-Fr
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-CD42b antibody [SP202]
See all CD42b primary antibodies -
Description
Rabbit monoclonal [SP202] to CD42b -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-Fr MouseRatIHC-P Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IHC-P: Human spleen, bone marrow, stomach adenocarcinoma and pancreatic adenocarcinoma tissue. IHC-Fr: Mouse and Rat spleen tissue.
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General notes
FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.1% Sodium azide
Constituents: 1% BSA, PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Monoclonal -
Clone number
SP202 -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Frozen) analysis of rat spleen tissue section labeling CD42b with purified ab227669. Sections were fixed in 4% paraformaldehyde and permeabilized with 0.2% Triton X-100. Antigen retrieval was heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody. DAPI was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
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Formalin-fixed, paraffin-embedded human spleen tissue stained for CD42b with ab227669 at 1/100 dilution in immunohistochemical analysis.
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Immunohistochemistry (Frozen) analysis of mouse spleen tissue section labeling CD42b with purified ab227669. Sections were fixed in 4% paraformaldehyde and permeabilized with 0.2% Triton X-100. Antigen retrieval was heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody. DAPI was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
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Formalin-fixed, paraffin-embedded human stomach adenocarcinoma tissue stained for CD42b with ab227669 at 1/100 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human pancreatic adenocarcinoma tissue stained for CD42b with ab227669 at 1/100 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human bone marrow tissue stained for CD42b with ab227669 at 1/100 dilution in immunohistochemical analysis.
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