Anti-STAT5 antibody (ab16276)
Key features and details
- Rabbit polyclonal to STAT5
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-STAT5 antibody
See all STAT5 primary antibodies -
Description
Rabbit polyclonal to STAT5 -
Host species
Rabbit -
Specificity
This antibody does not cross react with any other STATs or related proteins -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB Human
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Immunogen
Synthetic peptide:
ATIISEQQAKSLLKNENTR
, corresponding to amino acids 371-389 of Human STAT5. -
Positive control
- CTLL cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.3
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.1% BSA
PBS is Magnesium and Calcium free -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-STAT5 antibody (ab16276)Anti-STAT5 antibody (ab16276) + SK-BR-3 Human adenocarcinoma cell line
Secondary
HRP conjugated goat anti-rabbit
Predicted band size: 90 kDa
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Immunocytochemistry/ Immunofluorescence - Anti-STAT5 antibody (ab16276)ICC/IF image of ab71498 stained MCF7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab16276, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
