Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-STAT6 antibody [E265] - BSA and Azide free (ab247230)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [E265] to STAT6 - BSA and Azide free
  • Suitable for: ICC/IF, Flow Cyt (Intra), WB, IP
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-STAT6 antibody [E265] - BSA and Azide free
    See all STAT6 primary antibodies

  • Description

    Rabbit monoclonal [E265] to STAT6 - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    This antibody recognises Stat-6. It does not cross react with other STAT family members.

  • Tested applications

    Suitable for: ICC/IF, Flow Cyt (Intra), WB, IPmore details
    Unsuitable for: IHC

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Flow Cyt (intra): HeLa cells IP: HeLa whole cell lysate. WB: HeLa, Raji whole cell lysate.

  • General notes

    ab247230 is the carrier-free version of ab32108.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Images

  • Immunocytochemistry - Anti-STAT6 antibody [E265] - BSA and Azide free (ab247230)
    Immunocytochemistry - Anti-STAT6 antibody [E265] - BSA and Azide free (ab247230)
    This data was developed using ab32108, the same antibody clone in a different buffer formulation.Confocal image showing cytoplasmic staining in Raji cells. Immunocytochemistry/immunofluorescence analysis of Raji (human Burkitt's lymphoma B lymphocyte) cells labelling STAT6 with ab32108 at 10 µg/mL. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) was used as a secondary antibody at 2 µg/mL. Cells were counterstained with ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) at 1/200 dilution (red). Nuclear DNA was labelled with DAPI (blue).
  • Western blot - Anti-STAT6 antibody [E265] - BSA and Azide free (ab247230)
    Western blot - Anti-STAT6 antibody [E265] - BSA and Azide free (ab247230)

    This data was developed using ab32108, the same antibody clone in a different buffer formulation.

    Lane 1: Wild-type HAP1 whole cell lysate (20 µg)

    Lane 2: STAT6 knockout HAP1 whole cell lysate (20 µg)

    Lane 3: HeLa whole cell lysate (20 µg)

    Lane 4: Raji whole cell lysate (20 µg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab32108 observed at 94 kDa. Red - loading control, ab9484, observed at 37 kDa.

    ab32108 was shown to specifically react with STAT6 in wild-type HAP1 cells as signal was lost in STAT6 knockout cells. Wild-type and STAT6 knockout samples were subjected to SDS-PAGE. ab32108 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/5000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

  • Flow Cytometry - Anti-STAT6 antibody [E265] - BSA and Azide free (ab247230)
    Flow Cytometry - Anti-STAT6 antibody [E265] - BSA and Azide free (ab247230)
    This data was developed using ab32108, the same antibody clone in a different buffer formulation.
    Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling STAT6 with purified ab32108 at 1/100 dilution (10 µg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cells without incubation with primary antibody and secondary antibody (Blue).
  • Immunoprecipitation - Anti-STAT6 antibody [E265] - BSA and Azide free (ab247230)
    Immunoprecipitation - Anti-STAT6 antibody [E265] - BSA and Azide free (ab247230)

    This data was developed using ab32108, the same antibody clone in a different buffer formulation.
    Purified ab32108 at 1/50 dilution (2µg) immunoprecipitating STAT6 in HeLa whole cell lysate.
    Lane 1 (input): HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10µg
    Lane 2 (+): ab32108 + HeLa whole cell lysate.
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab32108 in HeLa whole cell lysate.
    VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
    Blocking Buffer and concentration: 5% NFDM/TBST.
    Diluting buffer and concentration: 5% NFDM/TBST.
    Observed band size: 100 kDa

  • Western blot - Anti-STAT6 antibody [E265] - BSA and Azide free (ab247230)
    Western blot - Anti-STAT6 antibody [E265] - BSA and Azide free (ab247230)
    Anti-STAT6 antibody [E265] (ab32108) at 1/5000 dilution
    Predicted band size: 94 kDa
    Observed band size: 100 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab32108, the same antibody clone in a different buffer formulation.

     

  • Anti-STAT6 antibody [E265] - BSA and Azide free (ab247230)
    Anti-STAT6 antibody [E265] - BSA and Azide free (ab247230)

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