Antibodies, Proteins, Kits and Reagents for Life Science

321 638 ₸ Product size

50 µl 321 638 ₸

Order now and get it on Wednesday March 03, 2021

Key features and details

Mouse monoclonal [9F7] to STAT5
Suitable for: WB, IHC-P, Flow Cyt, ICC/IF
Reacts with: Mouse, Rat, Dog, Human, African green monkey
Isotype: IgG1

Product name

Anti-STAT5 antibody [9F7]
See all STAT5 primary antibodies
Description

Mouse monoclonal [9F7] to STAT5
Host species

Mouse

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	African green monkey
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

Recombinant full length protein corresponding to Human STAT5.
Database link: P42229
Positive control
- WB: STAT5-transfected HEK-293T cell lysate; HepG2, HeLa, SVT2, COS-7, Jurkat, MDCK, PC-12 and MCF7 cell extracts. IHC-P: HUman testis tissue. ICC/IF: COS7 cells. Flow Cyt: HeLa and Jurkat cells.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA, 50% Glycerol
Concentration information loading...
Purity

Protein G purified
Clonality

Monoclonal
Clone number

9F7
Isotype

IgG1
Research areas

Western blot - Anti-STAT5 antibody [9F7] (ab230670)

All lanes : Anti-STAT5 antibody [9F7] (ab230670) at 1/500 dilution

Lane 1 : Untransfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Lane 2 : STAT5-transfected HEK-293T cell lysate

Lysates/proteins at 5 µg per lane.

Developed using the ECL technique.
Western blot - Anti-STAT5 antibody [9F7] (ab230670)

All lanes : Anti-STAT5 antibody [9F7] (ab230670) at 1/500 dilution

Lane 1 : HepG2 (human liver hepatocellular carcinoma cell line) cell extract
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell extract
Lane 3 : SVT2 cell extract
Lane 4 : A549 (human lung carcinoma cell line) cell extract
Lane 5 : COS-7 (African green monkey kidney fibroblast-like cell line) cell extract
Lane 6 : Jurkat (human T cell leukemia cell line from peripheral blood) cell extract
Lane 7 : MDCK (canine kidney cell line) cell extract
Lane 8 : PC-12 (rat adrenal gland pheochromocytoma cell line) cell extract
Lane 9 : MCF7 (human breast adenocarcinoma cell line) cell extract

Lysates/proteins at 35 µg per lane.

Developed using the ECL technique.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT5 antibody [9F7] (ab230670)

Formalin-fixed, paraffin-embedded human testis tissue stained for STAT5 with ab230670 at 10 μg/ml in immunohistochemical analysis.
Immunocytochemistry/ Immunofluorescence - Anti-STAT5 antibody [9F7] (ab230670)

COS-7 (African green monkey kidney fibroblast-like cell line) cells transiently transfected with STAT5 and stained for STAT5 (green) using ab230670 at 1/100 dilution.
Flow Cytometry - Anti-STAT5 antibody [9F7] (ab230670)

Flow cytometric analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling STAT% with ab230670 at 1/100 dilution (red) compared with a negative control (blue).
Flow Cytometry - Anti-STAT5 antibody [9F7] (ab230670)

Flow cytometric analysis of Jurkat (human T cell leukemia cell line from peripheral blood) cells labeling STAT% with ab230670 at 1/100 dilution (red) compared with a negative control (blue).

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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