Antibodies, Proteins, Kits and Reagents for Life Science

291 484 ₸ Product size

100 µl 291 484 ₸

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Anti-STAT6 antibody [EPR18278-222] (ab217998)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR18278-222] to STAT6
Suitable for: ICC/IF, WB, IP
Reacts with: Mouse, Rat, Human

Product name

Anti-STAT6 antibody [EPR18278-222]
See all STAT6 primary antibodies
Description

Rabbit monoclonal [EPR18278-222] to STAT6
Host species

Rabbit

Tested Applications & Species

Application	Species
ICC/IF	Human
IP	Human
WB	Mouse Rat Human

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Human thymus lysate; HeLa, P388D1, Ramos, THP-1, Daudi, Raji and A20 whole cell lysates; Mouse and rat spleen lysates. ICC/IF: Raji and Daudi cells. IP: Raji whole cell lysate.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR18278-222
Isotype

IgG
Research areas

Western blot - Anti-STAT6 antibody [EPR18278-222] (ab217998)

All lanes : Anti-STAT6 antibody [EPR18278-222] (ab217998) at 1/1000 dilution

Lane 1 : Human thymus lysate
Lane 2 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 3 : P388D1 (Mouse lymphoma N106 O91monocyte; macrophage cell line) whole cell lysate
Lane 4 : Ramos (Human Burkitt's lymphoma cell line) whole cell lysate
Lane 5 : THP-1 (Human monocytic leukemia cell line) whole cell lysate
Lane 6 : Daudi (Human Burkitt's lymphoma cell line) whole cell lysate
Lane 7 : Raji (Human Burkitt's lymphoma cell line) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 94 kDa
Observed band size: 110,75,81 kDa
why is the actual band size different from the predicted?

Blocking/Dilution buffer: 5% NFDM/TBST.

Exposure time: Lane 1,2 and 3: 8 seconds; Lane 4 and 5: 2 seconds; Lane 6 and 7: 1 second.

The molecular weight observed is consistent with what has been described in the literature (PMID 21411736). This antibody recognizes three isoforms of STAT6, isoform 1 (110kDa), isoform 2 (75kDa), isoform 3 (81kDa), which is consistent with the Uniprot annotation.
Immunocytochemistry/ Immunofluorescence - Anti-STAT6 antibody [EPR18278-222] (ab217998)

Immunofluorescent analysis of 100% methanol-fixed Raji (Human Burkitt's lymphoma cell line) cells labeling STAT6 with ab217998 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic and nuclear staining on Raji cell line [PMID: 11781246] [PMID: 23575898].

The nuclear counterstain is DAPI (blue). Tubulin is detected with ab195889 (Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594)) at 1/200 dilution (red).

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor^® 488) (ab150077) at 1/1000 dilution.
Immunocytochemistry/ Immunofluorescence - Anti-STAT6 antibody [EPR18278-222] (ab217998)

Immunofluorescent analysis of 100% methanol-fixed Daudi (Human Burkitt's lymphoma cell line) cells labeling STAT6 with ab217998 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic and nuclear staining on Daudi cell line.

The nuclear counterstain is DAPI (blue). Tubulin is detected with ab195889 (Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594)) at 1/200 dilution (red).

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor^® 488) (ab150077) at 1/1000 dilution.
Western blot - Anti-STAT6 antibody [EPR18278-222] (ab217998)

All lanes : Anti-STAT6 antibody [EPR18278-222] (ab217998) at 1/2000 dilution

Lane 1 : Mouse spleen lysate
Lane 2 : Rat spleen lysate
Lane 3 : A20 (Mouse reticulum sarcoma cell line) whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 94 kDa
Observed band size: 110,75,81 kDa why is the actual band size different from the predicted?

Blocking/Dilution buffer: 5% NFDM/TBST.

Exposure time: Lane 1:10 seconds; Lane 2:3 seconds; Lane 3:5 seconds.
Immunoprecipitation - Anti-STAT6 antibody [EPR18278-222] (ab217998)

STAT6 was immunoprecipitated from 0.35 mg of Raji (Human Burkitt's lymphoma cell line) whole cell lysate with ab217998 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab217998 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

Lane 1: Raji whole cell lysate, 10 µg (Input).

Lane 2: ab217998 IP in Raji whole cell lysate.

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab217998 in Raji whole cell lysate.

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 1 second.

This antibody recognizes three isoforms of STAT6, isoform 1 (110kDa), isoform 2 (75kDa), isoform 3 (81kDa), respectively which is consistent with the Uniprot annotation.
Anti-STAT6 antibody [EPR18278-222] (ab217998)

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