Antibodies, Proteins, Kits and Reagents for Life Science

304 886 ₸ Product size

100 µl 304 886 ₸

Order now and get it on Tuesday March 09, 2021

Anti-STAT6 antibody [EPR1912] (ab134940)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR1912] to STAT6
Suitable for: WB, ICC/IF
Knockout validated
Reacts with: Human

Product name

Anti-STAT6 antibody [EPR1912]
See all STAT6 primary antibodies
Description

Rabbit monoclonal [EPR1912] to STAT6
Host species

Rabbit
Tested Applications & Species

Application Species
ICC/IF
Human

WB
Human

See all applications and species data
Immunogen

Synthetic peptide within Human STAT6. The exact sequence is proprietary.
Positive control
- Daudi cells; Daudi, HeLa, Human lymph node and Raji cell lysates.
General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
ICC/IF	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR1912
Isotype

IgG
Research areas

Western blot - Anti-STAT6 antibody [EPR1912] (ab134940)

Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
Lane 2: STAT6 knockout HAP1 whole cell lysate (20 µg)
Lane 3: HeLa whole cell lysate (20 µg)
Lane 4: Raji whole cell lysate (20 µg)

Lanes 1 - 4: Merged signal (red and green). Green - ab134940 observed at 94 kDa. Red - loading control, ab9484, observed at 37 kDa.

ab134940 was shown to recognize STAT6 in wild-type HAP1 cells as signal was lost at the expected MW in STAT6 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and STAT6 knockout samples were subjected to SDS-PAGE. Ab134940 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
Immunocytochemistry/ Immunofluorescence - Anti-STAT6 antibody [EPR1912] (ab134940)

Immunofluorescence analysis of Daudi cells, labelling STAT6 using ab134940 at a 1/100 dilution (green). Cell nuclei are shown in blue.
Western blot - Anti-STAT6 antibody [EPR1912] (ab134940)

All lanes : Anti-STAT6 antibody [EPR1912] (ab134940) at 1/1000 dilution

Lane 1 : HeLa cell lysate
Lane 2 : Human lymph node lysate
Lane 3 : Daudi cell lysate
Lane 4 : Raji cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Standard HRP labelled goat anti-rabbit at 1/2000 dilution

Developed using the ECL technique.

Predicted band size: 94 kDa
Anti-STAT6 antibody [EPR1912] (ab134940)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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