Human Hemoglobin subunit alpha Matched Antibody Pair Kit (ab222265)
Key features and details
- Unlabeled capture antibody, biotin-labeled detection antibody and calibrated protein standard
- For economical ELISA and ELISA-based assay development
- Reacts with: Human
- Range: 500 pg/ml - 32000 pg/ml
Overview
-
Product name
Human Hemoglobin subunit alpha Matched Antibody Pair Kit
See all Hemoglobin subunit alpha kits -
Detection method
Colorimetric -
Assay type
ELISA set -
Sensitivity
24.3 pg/ml -
Range
500 pg/ml - 32000 pg/ml -
Species reactivity
Reacts with: Human -
Product overview
Human Hemoglobin subunit alpha Matched Antibody Pair Kits include a capture and a biotinylated detector antibody pair, along with a calibrated protein standard, suitable for sandwich ELISA. The Matched Antibody Pair Kit can be used to quantify native and recombinant human Hemoglobin subunit alpha.
Optimization of the kit reagents to sample type, immunoassay format or instrumentation may be required. Guidelines for use of this kit in a standard 96-well microplate sandwich ELISA using HRP/TMB system of colorimetric detection is described in this assay procedure for the purposes of quantification.
Protocol information and tips on the use of the Matched Antibody Pair kits for sandwich ELISA can be found on our website. An accessory pack can be purchased which includes buffer reagents required to perform 10 x 96-well plate sandwich ELISAs (ab210905).
For additional information on the performance of the antibody pair used in this kit, please see our equivalent SimpleStep ELISA kit ab219049. Please note that while the antibody pair is the same provided in the corresponding SimpleStep ELISA Kit, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits.
-
Tested applications
Suitable for: ELISAmore details -
Platform
Reagents
Properties
-
Storage instructions
Store at -20°C. Please refer to protocols. -
Components 10 x 96 tests 5 x 96 tests Human Hemoglobin subunit alpha Capture Antibody 2 x 50µg 1 x 50µg Human Hemoglobin subunit alpha Detector Antibody 2 x 12.5µg 1 x 12.5µg Human Hemoglobin subunit alpha Lyophilized Protein 2 vials 1 vial -
Research areas
-
Function
Involved in oxygen transport from the lung to the various peripheral tissues. -
Tissue specificity
Red blood cells. -
Involvement in disease
Heinz body anemias
Alpha-thalassemia
Alpha(0)-thalassemia is associated with non-immune hydrops fetalis, a generalized edema of the fetus with fluid accumulation in the body cavities due to non-immune causes. Non-immune hydrops fetalis is not a diagnosis in itself but a symptom, a feature of many genetic disorders, and the end-stage of a wide variety of disorders.
Hemoglobin H disease -
Sequence similarities
Belongs to the globin family. -
Post-translational
modificationsThe initiator Met is not cleaved in variant Thionville and is acetylated. - Information by UniProt
-
Alternative names
- Alpha 1 globin
- Alpha globin
- Alpha one globin
see all -
Database links
- Entrez Gene: 3039 Human
- Entrez Gene: 3040 Human
- Omim: 141800 Human
- Omim: 141850 Human
- SwissProt: P69905 Human
- Unigene: 449630 Human
- Unigene: 654744 Human
Images
-
Human Hemoglobin subunit alpha standard curve
Standard calibration curve. Background subtracted values are graphed.
-
Sandwich ELISA - Human Hemoglobin subunit alpha Matched Antibody Pair Kit (ab222265)To learn more about the advantages of recombinant antibodies see here.
