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Cardiovascular Blood Serum Proteins

Anti-alpha 1 Fetoprotein antibody [EPR9309] (ab169552)

Price and availability

335 040 ₸

Availability

Order now and get it on Tuesday March 02, 2021

Anti-alpha 1 Fetoprotein antibody [EPR9309] (ab169552)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR9309] to alpha 1 Fetoprotein
  • Suitable for: WB, IHC-P, ICC/IF, IP, Flow Cyt
  • Reacts with: Human

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Overview

  • Product name

    Anti-alpha 1 Fetoprotein antibody [EPR9309]
    See all alpha 1 Fetoprotein primary antibodies
  • Description

    Rabbit monoclonal [EPR9309] to alpha 1 Fetoprotein
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    IP
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Recombinant fragment within Human alpha 1 Fetoprotein. The exact sequence is proprietary.

  • Positive control

    • Human plasma, HepG2 and Human fetal liver lysates, Human moderately differentiated hepatocellular carcinoma and Human poorly differentiated hepatocellular carcinoma tissues, HepG2 cells
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR9309
  • Isotype

    IgG
  • Research areas

    • Cardiovascular
    • Blood
    • Serum Proteins
    • Cancer
    • Tumor biomarkers
    • Tumor antigens
    • Developmental Biology
    • Organogenesis
    • Gut development
    • Liver development
    • Kits/ Lysates/ Other
    • Kits
    • ELISA Kits
    • ELISA Kits
    • Cardiovascular ELISA kits

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Fetoprotein antibody [EPR9309] (ab169552)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Fetoprotein antibody [EPR9309] (ab169552)

    Immunohistochemical analysis of paraffin embedded human moderately differentiated hepatocellular carcinoma tissue labeling alpha 1 Fetoprotein with ab169552 antibody at 1/50.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Western blot - Anti-alpha 1 Fetoprotein antibody [EPR9309] (ab169552)
    Western blot - Anti-alpha 1 Fetoprotein antibody [EPR9309] (ab169552)
    All lanes : Anti-alpha 1 Fetoprotein antibody [EPR9309] (ab169552) at 1/1000 dilution

    Lane 1 : Human plasma lysate
    Lane 2 : HepG2 lysate
    Lane 3 : Human fetal liver lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 68 kDa
    Observed band size: 70 kDa
    why is the actual band size different from the predicted?

  • Immunocytochemistry/ Immunofluorescence - Anti-alpha 1 Fetoprotein antibody [EPR9309] (ab169552)
    Immunocytochemistry/ Immunofluorescence - Anti-alpha 1 Fetoprotein antibody [EPR9309] (ab169552)
    Immunofluorescent analysis of HepG2 cells labeling alpha 1 Fetoprotein with ab169552 at 1/100.
  • Immunoprecipitation - Anti-alpha 1 Fetoprotein antibody [EPR9309] (ab169552)
    Immunoprecipitation - Anti-alpha 1 Fetoprotein antibody [EPR9309] (ab169552)
    Immunoprecipitation. ab169552 at 1/1000 staining alpha 1 Fetoprotein in HepG2 cell lysate immunoprecipitated using ab169552 at 1/10. Predicted band size : 68 kDa.
  • Flow Cytometry - Anti-alpha 1 Fetoprotein antibody [EPR9309] (ab169552)
    Flow Cytometry - Anti-alpha 1 Fetoprotein antibody [EPR9309] (ab169552)

    Flow Cytometry analysis of HepG2 (human hepatocellular carcinoma) cells labeling alpha 1 Fetoprotein with purified ab169552 at 1/50 dilution (red). The secondary antibody was Goat anti rabbit IgG (Alexa Fluor® 488) at 1/2000 dilution. A Rabbit monoclonal IgG (Black) was used as the isotype control and cells without incubation with primary antibody and secondary antibody (Blue) were used as unlabeled control.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Fetoprotein antibody [EPR9309] (ab169552)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Fetoprotein antibody [EPR9309] (ab169552)

    Immunohistochemical analysis of paraffin embedded Human poorly differentiated hepatocellular carcinoma tissue labeling alpha 1 Fetoprotein with ab169552 antibody at 1/50.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Anti-alpha 1 Fetoprotein antibody [EPR9309] (ab169552)
    Anti-alpha 1 Fetoprotein antibody [EPR9309] (ab169552)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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