Anti-alpha 1 Fetoprotein antibody (ab46799)
Key features and details
- Rabbit polyclonal to alpha 1 Fetoprotein
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-alpha 1 Fetoprotein antibody
See all alpha 1 Fetoprotein primary antibodies -
Description
Rabbit polyclonal to alpha 1 Fetoprotein -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF or IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cat
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Immunogen
A synthetic peptide corresponding to residues in C-terminus of human AFP.
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Positive control
- HepG2 cell lysate; Human fetal liver tissue
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-alpha 1 Fetoprotein antibody (ab46799)All lanes : Anti-alpha 1 Fetoprotein antibody (ab46799) at 1 µg/ml
Lane 1 : HepG2 (Human) Whole Cell Lysate
Lane 2 : Human liver tissue lysate - total protein (ab29889)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed at 1/50000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 69 kDa
Observed band size: 73 kDa why is the actual band size different from the predicted?
Additional bands at: 58 kDa, 65 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 1 minuteThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab46799 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.
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Western blot - Anti-alpha 1 Fetoprotein antibody (ab46799)Anti-alpha 1 Fetoprotein antibody (ab46799) at 1/50000 dilution + HepG2 cell lysate at 10 µg
Secondary
Goat anti-rabbit HRP labeled, at 1/2000 dilution
Predicted band size: 69 kDa
Observed band size: 69 kDa
