Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-alpha 1 Fetoprotein antibody [EPAFP61] - Low endotoxin, Azide free (ab215379)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPAFP61] to alpha 1 Fetoprotein - Low endotoxin, Azide free
  • Suitable for: WB, IP, IHC-P, ICC, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-alpha 1 Fetoprotein antibody [EPAFP61] - Low endotoxin, Azide free
    See all alpha 1 Fetoprotein primary antibodies

  • Description

    Rabbit monoclonal [EPAFP61] to alpha 1 Fetoprotein - Low endotoxin, Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IP, IHC-P, ICC, Flow Cytmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant full length protein corresponding to Human alpha 1 Fetoprotein.
    Database link: P02771

  • Positive control

    • Flow Cyt: HepG2 cells; IP: HepG2 whole cell lysate; ICC: HepG2 cells; IHC-P: Human liver cancer tissue; WB: HepG2 whole cell lysate.

  • General notes

    ab215379 is a carrier-free antibody designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our Low endotoxin, azide-free formats have low endotoxin level (≤ 1 EU/ml, determined by the LAL assay) and are free from azide, to achieve consistent experimental results in functional assays.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Western blot - Anti-alpha 1 Fetoprotein antibody [EPAFP61] - Low endotoxin, Azide free (ab215379)
    Western blot - Anti-alpha 1 Fetoprotein antibody [EPAFP61] - Low endotoxin, Azide free (ab215379)
    Anti-alpha 1 Fetoprotein antibody [EPAFP61] (ab133617) at 1/10000 dilution (Purified) + HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate at 15 µg

    Secondary
    Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 68 kDa



    This data was developed using ab133617, the same antibody clone in a different buffer formulation.

  • Immunocytochemistry - Anti-alpha 1 Fetoprotein antibody [EPAFP61] - Low endotoxin, Azide free (ab215379)
    Immunocytochemistry - Anti-alpha 1 Fetoprotein antibody [EPAFP61] - Low endotoxin, Azide free (ab215379)
    This data was developed using ab133617, the same antibody clone in a different buffer formulation.
    Immunocytochemistry analysis of HepG2 (Human hepatocellular carcinoma epithelial cell) cells labeling alpha 1 Fetoprotein with Purified ab133617 at 1/250 dilution (0.52 µg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 dilution (2.5 µg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 dilution (2 µg/mL). DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Fetoprotein antibody [EPAFP61] - Low endotoxin, Azide free (ab215379)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Fetoprotein antibody [EPAFP61] - Low endotoxin, Azide free (ab215379)
    This data was developed using ab133617, the same antibody clone in a different buffer formulation.
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver cancer tissue sections labeling alpha 1 Fetoprotein with Purified ab133617 at 1/100 dilution (1.3 µg/mL). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 1 (pH 6.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  • Flow Cytometry - Anti-alpha 1 Fetoprotein antibody [EPAFP61] - Low endotoxin, Azide free (ab215379)
    Flow Cytometry - Anti-alpha 1 Fetoprotein antibody [EPAFP61] - Low endotoxin, Azide free (ab215379)
    This data was developed using ab133617, the same antibody clone in a different buffer formulation.
    Flow Cytometry analysis of HepG2 Human hepatocellular carcinoma epithelial cell cells labeling alpha 1 Fetoprotein with purified ab133617 at 1/100 dilution (10 µg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cells without incubation with primary antibody and secondary antibody (Blue).
  • Immunoprecipitation - Anti-alpha 1 Fetoprotein antibody [EPAFP61] - Low endotoxin, Azide free (ab215379)
    Immunoprecipitation - Anti-alpha 1 Fetoprotein antibody [EPAFP61] - Low endotoxin, Azide free (ab215379)

    This data was developed using ab133617, the same antibody clone in a different buffer formulation.
    Purified ab133617 at 1/20 dilution (0.5µg) immunoprecipitating alpha 1 Fetoprotein in HepG2 whole cell lysate.
    Lane 1 (input): HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate 10µg
    Lane 2 (+): ab133617 + HepG2 whole cell lysate.
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab133617 in HepG2 whole cell lysate.
    VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/5000 dilution) was used for Western blotting.
    Blocking Buffer and concentration: 5% NFDM/TBST.
    Diluting buffer and concentration: 5% NFDM/TBST.
    Observed band size: 70 kDa
    Lower bands could be caspase-dependent cleavage fragments. (PMID: 31811908)

  • Anti-alpha 1 Fetoprotein antibody [EPAFP61] - Low endotoxin, Azide free (ab215379)
    Anti-alpha 1 Fetoprotein antibody [EPAFP61] - Low endotoxin, Azide free (ab215379)

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