Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-alpha 1 Fetoprotein antibody [EPR19240-133] - BSA and Azide free (ab226488)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19240-133] to alpha 1 Fetoprotein - BSA and Azide free
  • Suitable for: WB, ICC/IF, IP
  • Reacts with: Mouse

Overview

  • Product name

    Anti-alpha 1 Fetoprotein antibody [EPR19240-133] - BSA and Azide free
    See all alpha 1 Fetoprotein primary antibodies

  • Description

    Rabbit monoclonal [EPR19240-133] to alpha 1 Fetoprotein - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ICC/IF, IPmore details

  • Species reactivity

    Reacts with: Mouse

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • ICC/IF: Hepa1-6 cells.

  • General notes

    Ab226488 is the carrier-free version of ab199054. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab226488 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunoprecipitation - Anti-alpha 1 Fetoprotein antibody [EPR19240-133] - BSA and Azide free (ab226488)
    Immunoprecipitation - Anti-alpha 1 Fetoprotein antibody [EPR19240-133] - BSA and Azide free (ab226488)

    alpha 1 Fetoprotein was immunoprecipitated from 0.35 mg of mouse placenta lysate with ab199054 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab199054 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

    Lane 1: Mouse placenta lysate 10 µg (Input). 

    Lane 2: ab199054 IP in mouse placenta lysate. 

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab199054 in mouse placenta lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 1 second.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab199054).

  • Immunocytochemistry/ Immunofluorescence - Anti-alpha 1 Fetoprotein antibody [EPR19240-133] - BSA and Azide free (ab226488)
    Immunocytochemistry/ Immunofluorescence - Anti-alpha 1 Fetoprotein antibody [EPR19240-133] - BSA and Azide free (ab226488)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Hepa1-6 (mouse hepatoma epithelial cell line) cells labeling alpha 1 Fetoprotein with ab199054 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on Hepa1-6 cells.

    The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab199054).

  • Anti-alpha 1 Fetoprotein antibody [EPR19240-133] - BSA and Azide free (ab226488)
    Anti-alpha 1 Fetoprotein antibody [EPR19240-133] - BSA and Azide free (ab226488)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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