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Anti-alpha 1 Fetoprotein antibody [EPAFP61] (ab133617)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPAFP61] to alpha 1 Fetoprotein
  • Suitable for: WB, IHC-P, IP, Flow Cyt, ICC
  • Reacts with: Human

Overview

  • Product name

    Anti-alpha 1 Fetoprotein antibody [EPAFP61]
    See all alpha 1 Fetoprotein primary antibodies

  • Description

    Rabbit monoclonal [EPAFP61] to alpha 1 Fetoprotein

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC
    Human
    IHC-P
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant full length protein corresponding to Human alpha 1 Fetoprotein.
    Database link: P02771

  • Positive control

    • Flow Cyt: HepG2 cells; IP: HepG2 whole cell lysate; ICC: HepG2 cells; IHC-P: Human liver cancer tissue; WB: HepG2 whole cell lysate.

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-alpha 1 Fetoprotein antibody [EPAFP61] (ab133617)
    Western blot - Anti-alpha 1 Fetoprotein antibody [EPAFP61] (ab133617)
    Anti-alpha 1 Fetoprotein antibody [EPAFP61] (ab133617) at 1/10000 dilution (Purified) + HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate at 15 µg

    Secondary
    Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 68 kDa

  • Immunocytochemistry - Anti-alpha 1 Fetoprotein antibody [EPAFP61] (ab133617)
    Immunocytochemistry - Anti-alpha 1 Fetoprotein antibody [EPAFP61] (ab133617)
    Immunocytochemistry analysis of HepG2 (Human hepatocellular carcinoma epithelial cell) cells labeling alpha 1 Fetoprotein with Purified ab133617 at 1/250 dilution (0.52 µg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 dilution (2.5 µg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 dilution (2 µg/mL). DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Fetoprotein antibody [EPAFP61] (ab133617)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Fetoprotein antibody [EPAFP61] (ab133617)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver cancer tissue sections labeling alpha 1 Fetoprotein with Purified ab133617 at 1/100 dilution (1.3 µg/mL). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 1 (pH 6.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  • Immunoprecipitation - Anti-alpha 1 Fetoprotein antibody [EPAFP61] (ab133617)
    Immunoprecipitation - Anti-alpha 1 Fetoprotein antibody [EPAFP61] (ab133617)

    Purified ab133617 at 1/20 dilution (0.5µg) immunoprecipitating alpha 1 Fetoprotein in HepG2 whole cell lysate.
    Lane 1 (input): HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate 10µg
    Lane 2 (+): ab133617 + HepG2 whole cell lysate.
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab133617 in HepG2 whole cell lysate.
    VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/5000 dilution) was used for Western blotting.
    Blocking Buffer and concentration: 5% NFDM/TBST.
    Diluting buffer and concentration: 5% NFDM/TBST.
    Observed band size: 70 kDa
    Lower bands could be caspase-dependent cleavage fragments. (PMID: 31811908)

  • Flow Cytometry - Anti-alpha 1 Fetoprotein antibody [EPAFP61] (ab133617)
    Flow Cytometry - Anti-alpha 1 Fetoprotein antibody [EPAFP61] (ab133617)
    Flow Cytometry analysis of HepG2 Human hepatocellular carcinoma epithelial cell cells labeling alpha 1 Fetoprotein with purified ab133617 at 1/100 dilution (10 µg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cells without incubation with primary antibody and secondary antibody (Blue).
  • Anti-alpha 1 Fetoprotein antibody [EPAFP61] (ab133617)
    Anti-alpha 1 Fetoprotein antibody [EPAFP61] (ab133617)

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