Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Angiotensin II Type 2 Receptor antibody [EPR3876] (ab92445)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3876] to Angiotensin II Type 2 Receptor
  • Suitable for: WB, IP
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Angiotensin II Type 2 Receptor antibody [EPR3876]
    See all Angiotensin II Type 2 Receptor primary antibodies

  • Description

    Rabbit monoclonal [EPR3876] to Angiotensin II Type 2 Receptor

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Mouse
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human Angiotensin II Type 2 Receptor aa 300-400 (C terminal). The exact sequence is proprietary.

  • Positive control

    • WB: HepG2, Human heart, Mouse heart, and Rat heart lysates. IP: Mouse heart cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-Angiotensin II Type 2 Receptor antibody [EPR3876] (ab92445)
    Western blot - Anti-Angiotensin II Type 2 Receptor antibody [EPR3876] (ab92445)
    All lanes : Anti-Angiotensin II Type 2 Receptor antibody [EPR3876] (ab92445) at 1/1000 dilution (Purified)

    Lane 1 : HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysates
    Lane 2 : Human heart lysates
    Lane 3 : Mouse heart lysates
    Lane 4 : Rat heart lysates

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 41 kDa
    Observed band size: 41 kDa

  • Immunoprecipitation - Anti-Angiotensin II Type 2 Receptor antibody [EPR3876] (ab92445)
    Immunoprecipitation - Anti-Angiotensin II Type 2 Receptor antibody [EPR3876] (ab92445)

    ab92445 (purified ) at 1/20 dilution (0.2ug) immunoprecipitating Angiotensin II Type 2 Receptor in Mouse heart lysate .
    Lane 1 (input): Mouse heart lysate 10ug
    Lane 2 (+): ab92445 & Mouse heart lysate
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab92445 in Mouse heart lysate
    For western blotting, VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/1000 dilution.
    Blocking and diluting buffer: 5% NFDM/TBST.

  • Anti-Angiotensin II Type 2 Receptor antibody [EPR3876] (ab92445)
    Anti-Angiotensin II Type 2 Receptor antibody [EPR3876] (ab92445)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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