Anti-alpha 1 Fetoprotein antibody [EPR20667] - BSA and Azide free (ab238439)
![Anti-alpha 1 Fetoprotein antibody [EPR20667] - BSA and Azide free (ab238439)](https://www.abcam.com/ps/products/238/ab238439/Images/ab238439-2-ab213328280318213328IF.jpg "Anti-alpha 1 Fetoprotein antibody [EPR20667] - BSA and Azide free (ab238439)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR20667] to alpha 1 Fetoprotein - BSA and Azide free
- Suitable for: Flow Cyt, WB, ICC/IF
- Reacts with: Mouse, Rat
Overview
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Product name
Anti-alpha 1 Fetoprotein antibody [EPR20667] - BSA and Azide free
See all alpha 1 Fetoprotein primary antibodies -
Description
Rabbit monoclonal [EPR20667] to alpha 1 Fetoprotein - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt, WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
(Peptide available asab188828) -
Positive control
- ICC/IF: Hepa1-6 cells. Flow Cyt: Hepa1-6 cells.
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General notes
Ab238439 is the carrier-free version of ab213328. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab238439 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR20667 -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-alpha 1 Fetoprotein antibody [EPR20667] - BSA and Azide free (ab238439)
Immunofluorescent analysis of 4% paraformaldehyde-fixed. 0.1% Triton X-100 permeabilized Hepa1-6 (mouse hepatoma epithelial cell line) cells labeling alpha 1 Fetoprotein with ab213328 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on Hepa1-6 cell line.
The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol and sodium azide (ab213328)
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![Flow Cytometry - Anti-alpha 1 Fetoprotein antibody [EPR20667] - BSA and Azide free (ab238439)](https://www.abcam.com/ps/products/238/ab238439/Images/ab238439-1-ab213328280317213328FC.jpg)
Flow Cytometry - Anti-alpha 1 Fetoprotein antibody [EPR20667] - BSA and Azide free (ab238439)Flow cytometric analysis of 4% paraformaldehyde-fixed Hepa1-6 (mouse hepatoma epithelial cell line) cell line labeling alpha 1 Fetoprotein with ab213328 at 1/500 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/2000 dilution was used as the secondary antibody.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol and sodium azide (ab213328)
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![Anti-alpha 1 Fetoprotein antibody [EPR20667] - BSA and Azide free (ab238439)](https://www.abcam.com/ps/products/238/ab238439/Images/ab238439-3-benefits-of-recombinant-antibodies.png)
Anti-alpha 1 Fetoprotein antibody [EPR20667] - BSA and Azide free (ab238439)
