Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-alpha 1 Fetoprotein antibody [EPR20667] - BSA and Azide free (ab238439)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR20667] to alpha 1 Fetoprotein - BSA and Azide free
  • Suitable for: Flow Cyt, WB, ICC/IF
  • Reacts with: Mouse, Rat

Overview

  • Product name

    Anti-alpha 1 Fetoprotein antibody [EPR20667] - BSA and Azide free
    See all alpha 1 Fetoprotein primary antibodies

  • Description

    Rabbit monoclonal [EPR20667] to alpha 1 Fetoprotein - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt, WB, ICC/IFmore details

  • Species reactivity

    Reacts with: Mouse, Rat

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
    (Peptide available as ab188828)

  • Positive control

    • ICC/IF: Hepa1-6 cells. Flow Cyt: Hepa1-6 cells.

  • General notes

    Ab238439 is the carrier-free version of ab213328. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab238439 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-alpha 1 Fetoprotein antibody [EPR20667] - BSA and Azide free (ab238439)
    Immunocytochemistry/ Immunofluorescence - Anti-alpha 1 Fetoprotein antibody [EPR20667] - BSA and Azide free (ab238439)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed. 0.1% Triton X-100 permeabilized Hepa1-6 (mouse hepatoma epithelial cell line) cells labeling alpha 1 Fetoprotein with ab213328 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on Hepa1-6 cell line.

    The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol and sodium azide (ab213328)

  • Flow Cytometry - Anti-alpha 1 Fetoprotein antibody [EPR20667] - BSA and Azide free (ab238439)
    Flow Cytometry - Anti-alpha 1 Fetoprotein antibody [EPR20667] - BSA and Azide free (ab238439)

    Flow cytometric analysis of 4% paraformaldehyde-fixed Hepa1-6 (mouse hepatoma epithelial cell line) cell line labeling alpha 1 Fetoprotein with ab213328 at 1/500 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/2000 dilution was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol and sodium azide (ab213328)

  • Anti-alpha 1 Fetoprotein antibody [EPR20667] - BSA and Azide free (ab238439)
    Anti-alpha 1 Fetoprotein antibody [EPR20667] - BSA and Azide free (ab238439)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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