Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-XBP1 antibody [EPR22004] - BSA and Azide free (ab238456)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22004] to XBP1 - BSA and Azide free
  • Suitable for: WB, Flow Cyt, IP
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-XBP1 antibody [EPR22004] - BSA and Azide free
    See all XBP1 primary antibodies

  • Description

    Rabbit monoclonal [EPR22004] to XBP1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, Flow Cyt, IPmore details

  • Species reactivity

    Reacts with: Mouse, Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Flow Cyt: Huh7 treated with 300 nM Thapsigargin for 18 hours.

  • General notes

    Ab238456 is the carrier-free version of ab220783. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab238456 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunoprecipitation - Anti-XBP1 antibody [EPR22004] - BSA and Azide free (ab238456)
    Immunoprecipitation - Anti-XBP1 antibody [EPR22004] - BSA and Azide free (ab238456)

    XBP1 was immunoprecipitated from 0.35 mg Huh7 (human hepatocellular carcinoma epithelial cell line) treated with 300 nM Thapsigargin for 18 hours whole cell lysate with ab220783 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab220783 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.

    Lane 1: Huh7 treated with 300 nM Thapsigargin for 18 hours whole cell lysate 10 µg (Input).
    Lane 2: ab220783 IP in Huh7 treated with 300 nM Thapsigargin for 18 hours whole cell lysate (+).
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab220783 in Huh7 treated with 300 nM Thapsigargin for 18 hours whole cell lysate (-).

    Blocking/Dilution buffer: 5% NFDM/TBST.
    Exposure time: 3 minutes.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab220783).

  • Flow Cytometry - Anti-XBP1 antibody [EPR22004] - BSA and Azide free (ab238456)
    Flow Cytometry - Anti-XBP1 antibody [EPR22004] - BSA and Azide free (ab238456)

    Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized Huh7 (human hepatocellular carcinoma epithelial cell line) cells treated with 300 nM Thapsigargin for 18 hours (red) / Untreated control (green) labeling XBP1 with ab220783 at 1/100 dilution compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells incubated with secondary antibody only) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol and sodium azide (ab220783).

  • Anti-XBP1 antibody [EPR22004] - BSA and Azide free (ab238456)
    Anti-XBP1 antibody [EPR22004] - BSA and Azide free (ab238456)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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