Anti-BZW1/Protein Orf antibody (ab229314)
Key features and details
- Rabbit polyclonal to BZW1/Protein Orf
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-BZW1/Protein Orf antibody
See all BZW1/Protein Orf primary antibodies -
Description
Rabbit polyclonal to BZW1/Protein Orf -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Chicken, Cow, Pig, Xenopus laevis
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Immunogen
Recombinant fragment within Human BZW1/Protein Orf (internal sequence). The exact sequence is proprietary.
Database link: Q7L1Q6 -
Positive control
- ICC/IF: A431 cells. WB: Mouse brain lysate; rat brain lysate; U87 MG, SK-N-SH, IMR32 and SK-N-AS whole cell extracts.
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General notes
This product was previously labelled as BZW1
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 79.99% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-BZW1/Protein Orf antibody (ab229314)
A431 (Human epidermoid carcinoma cell line) cells stained for BZW1/Protein Orf (green) using ab229314 (1/500 dilution) in ICC/IF. Cells were fixed in 4% paraformaldehyde at RT for 15 minutes.
Nuclear counterstain: Hoechst 33342 (blue).
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Western blot - Anti-BZW1/Protein Orf antibody (ab229314)All lanes : Anti-BZW1/Protein Orf antibody (ab229314) at 1/500 dilution
Lane 1 : U-87 MG (human glioblastoma-astrocytoma epithelial cell line) whole cell extract
Lane 2 : SK-N-SH (human neuroblastoma cell line) whole cell extract
Lane 3 : IMR32 whole cell extract
Lane 4 : SK-N-AS whole cell extract
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 48 kDa
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Western blot - Anti-BZW1/Protein Orf antibody (ab229314)All lanes : Anti-BZW1/Protein Orf antibody (ab229314) at 1/1000 dilution
Lane 1 : Mouse brain lysate
Lane 2 : Rat brain lysate
Lysates/proteins at 50 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 48 kDa
