Antibodies, Proteins, Kits and Reagents for Life Science

278 083 ₸ Product size

100 µl 278 083 ₸

Order now and get it on Wednesday March 03, 2021

Key features and details

Mouse monoclonal [OTI3H9] to PANK2
Suitable for: WB, IHC-P, Flow Cyt, ICC/IF
Reacts with: Rat, Dog, Human, African green monkey
Isotype: IgG2a

Product name

Anti-PANK2 antibody [OTI3H9]
See all PANK2 primary antibodies
Description

Mouse monoclonal [OTI3H9] to PANK2
Host species

Mouse

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	African green monkey
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

Recombinant full length protein corresponding to Human PANK2 aa 1-600. Produced in HEK-293T cells (NP_705902).
Database link: Q9BZ23

Run BLAST with Run BLAST with
Positive control
- WB: HEK-293T cell lysate transfected with pCMV6-ENTRY PANK2 cDNA; HepG2, HeLa, HT-29, A549, COS-7, Jurkat, MDCK, PC-12, and MCF7 cell extracts. IHC-P: Human liver, prostate and prostate carcinoma tissues. ICC/IF: COS-7 cells transiently transfected by pCMV6-ENTRY PANK2 cDNA. Flow Cyt: Jurkat cells; HEK-293T cells transfected with a pCMV6-ENTRY PANK2 overexpress plasmid.
General notes

Clone OTI3H9 (formerly 3H9).

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 1% BSA
Concentration information loading...
Purity

Affinity purified
Purification notes

Purified from cell culture supernatant by affinity chromatography.
Clonality

Monoclonal
Clone number

OTI3H9
Isotype

IgG2a
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PANK2 antibody [OTI3H9] (ab119070)

Paraffin-embedded human liver tissue stained for PANK2 with ab119070 at a 1/50 dilution in immunohistochemical analysis. Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 minutes.
Western blot - Anti-PANK2 antibody [OTI3H9] (ab119070)

All lanes : Anti-PANK2 antibody [OTI3H9] (ab119070) at 1/1000 dilution

Lane 1 : HepG2 (Human liver hepatocellular carcinoma cell line) cell extract
Lane 2 : HeLa (Human epithelial cell line from cervix adenocarcinoma) cell extract
Lane 3 : HT-29 (Human colorectal adenocarcinoma cell line) cell extract
Lane 4 : A549 (Human lung carcinoma cell line) cell extract
Lane 5 : COS-7 (African green monkey kidney fibroblast-like cell line) cell extract
Lane 6 : Jurkat (Human T cell leukemia cell line from peripheral blood) cell extract
Lane 7 : MDCK (Canine kidney cell line) cell extract
Lane 8 : PC-12 (Rat adrenal gland pheochromocytoma cell line) cell extract
Lane 9 : MCF7 (Human breast adenocarcinoma cell line) cell extract

Lysates/proteins at 35 µg per lane.

Predicted band size: 63 kDa
Immunocytochemistry/ Immunofluorescence - Anti-PANK2 antibody [OTI3H9] (ab119070)

pCMV6-ENTRY PANK2 cDNA transfected COS-7 (African green monkey kidney fibroblast-like cell line) cells stained for PANK2 using ab119070 at a 1/100 dilution in ICC/IF.
Flow Cytometry - Anti-PANK2 antibody [OTI3H9] (ab119070)

ab119070 at 1/100 dilution staining PANK2 in Jurkat (Human T cell leukemia cell line from peripheral blood) cells by Flow cytometry (Red) compared to a nonspecific negative control antibody (Blue).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PANK2 antibody [OTI3H9] (ab119070)

Paraffin-embedded human prostate tissue stained for PANK2 with ab119070 at a 1/50 dilution in immunohistochemical analysis. Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 minutes.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PANK2 antibody [OTI3H9] (ab119070)

Paraffin-embedded human prostate carcinoma tissue stained for PANK2 with ab119070 at a 1/50 dilution in immunohistochemical analysis. Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 minutes.
Flow Cytometry - Anti-PANK2 antibody [OTI3H9] (ab119070)

ab119070 at 1/100 dilution staining PANK2 in HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cells transfected with either pCMV6-ENTRY PANK2 overexpress plasmid (Red) or empty vector control plasmid (Blue) by flow cytometry.
Western blot - Anti-PANK2 antibody [OTI3H9] (ab119070)

All lanes : Anti-PANK2 antibody [OTI3H9] (ab119070) at 1/1000 dilution

Lane 1 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate transfected with pCMV6-ENTRY control cDNA
Lane 2 : HEK-293T cell lysate transfected with pCMV6-ENTRY PANK2 cDNA

Lysates/proteins at 5 µg per lane.

Predicted band size: 63 kDa

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