Anti-PANK2 antibody [OTI3H9] (ab119070)
![Anti-PANK2 antibody [OTI3H9] (ab119070)](https://www.abcam.com/ps/products/119/ab119070/Images/PANK2-Primary-antibodies-ab119070-4.jpg "Anti-PANK2 antibody [OTI3H9] (ab119070)")
Key features and details
- Mouse monoclonal [OTI3H9] to PANK2
- Suitable for: WB, IHC-P, Flow Cyt, ICC/IF
- Reacts with: Rat, Dog, Human, African green monkey
- Isotype: IgG2a
Overview
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Product name
Anti-PANK2 antibody [OTI3H9]
See all PANK2 primary antibodies -
Description
Mouse monoclonal [OTI3H9] to PANK2 -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanICC/IF African green monkeyIHC-P HumanWB Human
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Immunogen
Recombinant full length protein corresponding to Human PANK2 aa 1-600. Produced in HEK-293T cells (NP_705902).
Database link: Q9BZ23 -
Positive control
- WB: HEK-293T cell lysate transfected with pCMV6-ENTRY PANK2 cDNA; HepG2, HeLa, HT-29, A549, COS-7, Jurkat, MDCK, PC-12, and MCF7 cell extracts. IHC-P: Human liver, prostate and prostate carcinoma tissues. ICC/IF: COS-7 cells transiently transfected by pCMV6-ENTRY PANK2 cDNA. Flow Cyt: Jurkat cells; HEK-293T cells transfected with a pCMV6-ENTRY PANK2 overexpress plasmid.
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General notes
Clone OTI3H9 (formerly 3H9).
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 1% BSA -
Concentration information loading... -
Purity
Affinity purified -
Purification notes
Purified from cell culture supernatant by affinity chromatography. -
Clonality
Monoclonal -
Clone number
OTI3H9 -
Isotype
IgG2a -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PANK2 antibody [OTI3H9] (ab119070)
Paraffin-embedded human liver tissue stained for PANK2 with ab119070 at a 1/50 dilution in immunohistochemical analysis. Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 minutes.
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![Western blot - Anti-PANK2 antibody [OTI3H9] (ab119070)](https://www.abcam.com/ps/products/119/ab119070/Images/PANK2-Primary-antibodies-ab119070-3.jpg)
Western blot - Anti-PANK2 antibody [OTI3H9] (ab119070)All lanes : Anti-PANK2 antibody [OTI3H9] (ab119070) at 1/1000 dilution
Lane 1 : HepG2 (Human liver hepatocellular carcinoma cell line) cell extract
Lane 2 : HeLa (Human epithelial cell line from cervix adenocarcinoma) cell extract
Lane 3 : HT-29 (Human colorectal adenocarcinoma cell line) cell extract
Lane 4 : A549 (Human lung carcinoma cell line) cell extract
Lane 5 : COS-7 (African green monkey kidney fibroblast-like cell line) cell extract
Lane 6 : Jurkat (Human T cell leukemia cell line from peripheral blood) cell extract
Lane 7 : MDCK (Canine kidney cell line) cell extract
Lane 8 : PC-12 (Rat adrenal gland pheochromocytoma cell line) cell extract
Lane 9 : MCF7 (Human breast adenocarcinoma cell line) cell extract
Lysates/proteins at 35 µg per lane.
Predicted band size: 63 kDa
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![Immunocytochemistry/ Immunofluorescence - Anti-PANK2 antibody [OTI3H9] (ab119070)](https://www.abcam.com/ps/products/119/ab119070/Images/PANK2-Primary-antibodies-ab119070-7.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-PANK2 antibody [OTI3H9] (ab119070)pCMV6-ENTRY PANK2 cDNA transfected COS-7 (African green monkey kidney fibroblast-like cell line) cells stained for PANK2 using ab119070 at a 1/100 dilution in ICC/IF.
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![Flow Cytometry - Anti-PANK2 antibody [OTI3H9] (ab119070)](https://www.abcam.com/ps/products/119/ab119070/Images/PANK2-Primary-antibodies-ab119070-9.jpg)
Flow Cytometry - Anti-PANK2 antibody [OTI3H9] (ab119070)ab119070 at 1/100 dilution staining PANK2 in Jurkat (Human T cell leukemia cell line from peripheral blood) cells by Flow cytometry (Red) compared to a nonspecific negative control antibody (Blue).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PANK2 antibody [OTI3H9] (ab119070)](https://www.abcam.com/ps/products/119/ab119070/Images/PANK2-Primary-antibodies-ab119070-5.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PANK2 antibody [OTI3H9] (ab119070)Paraffin-embedded human prostate tissue stained for PANK2 with ab119070 at a 1/50 dilution in immunohistochemical analysis. Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 minutes.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PANK2 antibody [OTI3H9] (ab119070)](https://www.abcam.com/ps/products/119/ab119070/Images/PANK2-Primary-antibodies-ab119070-6.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PANK2 antibody [OTI3H9] (ab119070)Paraffin-embedded human prostate carcinoma tissue stained for PANK2 with ab119070 at a 1/50 dilution in immunohistochemical analysis. Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 minutes.
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![Flow Cytometry - Anti-PANK2 antibody [OTI3H9] (ab119070)](https://www.abcam.com/ps/products/119/ab119070/Images/PANK2-Primary-antibodies-ab119070-8.jpg)
Flow Cytometry - Anti-PANK2 antibody [OTI3H9] (ab119070)ab119070 at 1/100 dilution staining PANK2 in HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cells transfected with either pCMV6-ENTRY PANK2 overexpress plasmid (Red) or empty vector control plasmid (Blue) by flow cytometry.
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![Western blot - Anti-PANK2 antibody [OTI3H9] (ab119070)](https://www.abcam.com/ps/products/119/ab119070/Images/PANK2-Primary-antibodies-ab119070-1.jpg)
Western blot - Anti-PANK2 antibody [OTI3H9] (ab119070)All lanes : Anti-PANK2 antibody [OTI3H9] (ab119070) at 1/1000 dilution
Lane 1 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate transfected with pCMV6-ENTRY control cDNA
Lane 2 : HEK-293T cell lysate transfected with pCMV6-ENTRY PANK2 cDNA
Lysates/proteins at 5 µg per lane.
Predicted band size: 63 kDa
