Anti-XBP1 antibody (ab37151)
Key features and details
- Rabbit polyclonal to XBP1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-XBP1 antibody
See all XBP1 primary antibodies -
Description
Rabbit polyclonal to XBP1 -
Host species
Rabbit -
Specificity
This product will recognize only the non-spliced (shorter) form of XBP1. -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide corresponding to 17 amino acids near to the C terminus of the human XBP1 protein.
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Positive control
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-XBP1 antibody (ab37151)Western blot analysis of XBP-1 in PC-3 cell lysate with ab37151 at (A) 0.5, (B) 1 and (C) 2 µg/ml.
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Western blot - Anti-XBP1 antibody (ab37151)All lanes : Anti-XBP1 antibody (ab37151) at 1 µg/ml
Lane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 2 : Human placenta tissue lysate - total protein (ab29745)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 29 kDa
Observed band size: 42 kDa why is the actual band size different from the predicted?
Additional bands at: 29 kDa, 86 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 8 minutes
The 42 kDa band observed is comparable to the molecular weight seen with other commercially available antibodies to XBP1.
