Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-XBP1 antibody [EPR22004] (ab220783)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22004] to XBP1
  • Suitable for: Flow Cyt, IP, WB
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-XBP1 antibody [EPR22004]
    See all XBP1 primary antibodies

  • Description

    Rabbit monoclonal [EPR22004] to XBP1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IP
    Human
    WB
    Mouse
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Huh7 treated with 300 nM thapsigargin for 18 hours, whole cell lysate; RAW 264.7 treated with 300 nM thapsigargin for 18 hours, whole cell lysate; U266B1, MCF7, HEK-293T and NIH/3T3 whole cell lysates. Flow Cyt: Huh7 treated with 300 nM Thapsigargin for 18 hours. IP: Huh7 treated with 300 nM Thapsigargin for 18 hours whole cell lysate.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-XBP1 antibody [EPR22004] (ab220783)
    Western blot - Anti-XBP1 antibody [EPR22004] (ab220783)
    All lanes : Anti-XBP1 antibody [EPR22004] (ab220783) at 1/1000 dilution

    Lane 1 : Untreated Huh7 (human hepatocellular carcinoma epithelial cell line) whole cell lysate
    Lane 2 : Huh7 treated with 300 nM thapsigargin for 18 hours, whole cell lysate
    Lane 3 : Untreated RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
    Lane 4 : RAW 264.7 treated with 300 nM thapsigargin for 18 hours, whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 29 kDa
    Observed band size: 29,56 kDa
    why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

    Spliced XBP1 (XBP1s) is induced after ER stress which is mimicked by treatment with thapsigargin. The expression profile observed is consistent with what has been described in the literature (PMID:11779464; PMID 25190803).

  • Flow Cytometry - Anti-XBP1 antibody [EPR22004] (ab220783)
    Flow Cytometry - Anti-XBP1 antibody [EPR22004] (ab220783)

    Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized Huh7 (human hepatocellular carcinoma epithelial cell line) cells treated with 300 nM Thapsigargin for 18 hours (red) / Untreated control (green) labeling XBP1 with ab220783 at 1/100 dilution compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells incubated with secondary antibody only) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody.

  • Immunoprecipitation - Anti-XBP1 antibody [EPR22004] (ab220783)
    Immunoprecipitation - Anti-XBP1 antibody [EPR22004] (ab220783)

    XBP1 was immunoprecipitated from 0.35 mg Huh7 (human hepatocellular carcinoma epithelial cell line) treated with 300 nM Thapsigargin for 18 hours whole cell lysate with ab220783 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab220783 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.

    Lane 1: Huh7 treated with 300 nM Thapsigargin for 18 hours whole cell lysate 10 µg (Input).
    Lane 2: ab220783 IP in Huh7 treated with 300 nM Thapsigargin for 18 hours whole cell lysate (+).
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab220783 in Huh7 treated with 300 nM Thapsigargin for 18 hours whole cell lysate (-).

    Blocking/Dilution buffer: 5% NFDM/TBST.
    Exposure time: 3 minutes.

  • Western blot - Anti-XBP1 antibody [EPR22004] (ab220783)
    Western blot - Anti-XBP1 antibody [EPR22004] (ab220783)
    All lanes : Anti-XBP1 antibody [EPR22004] (ab220783) at 1/1000 dilution

    Lane 1 : U266B1 (human multiple myeloma B lymphocyte cell line) whole cell lysate
    Lane 2 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate
    Lane 3 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 4 : NIH/3T3 (mouse embyro fibroblast cell line) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 29 kDa
    Observed band size: 29,56 kDa why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

    The expression profile observed is consistent with what has been described in the literature (PMID: 11779464; PMID 25190803).

  • Anti-XBP1 antibody [EPR22004] (ab220783)
    Anti-XBP1 antibody [EPR22004] (ab220783)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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