Antibodies, Proteins, Kits and Reagents for Life Science

314 937 ₸ Product size

100 µg 314 937 ₸ 10 µg 107 212 ₸

Order now and get it on Tuesday March 09, 2021

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Mouse monoclonal [143F] to XBP1
Suitable for: WB, IHC-P, ICC/IF
Reacts with: Human

Product name

Anti-XBP1 antibody [143F]
See all XBP1 primary antibodies
Description

Mouse monoclonal [143F] to XBP1
Host species

Mouse

Tested Applications & Species

Application	Species
ICC/IF	Human
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

Recombinant fragment. This information is considered to be commercially sensitive.
Positive control
- WB: Huh7+ thapsigargin (300nM 18h) lysate. IHC-P: Human non-Hodgkin's lymphoma and tonsil tissue. ICC/IF: Huh7 cells treated with thapsigargin (300 nM) for 18 hours.
General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

143F
Isotype

IgG2a
Research areas

Western blot - Anti-XBP1 antibody [143F] (ab241571)

All lanes : Anti-XBP1 antibody [143F] (ab241571) at 1/1000 dilution

Lane 1 : Huh7+ thapsigargin (300nM 18h) (-) lysate
Lane 2 : Huh7+ thapsigargin (300nM 18h) (+) lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Mouse IgG, (H+L), Peroxidase conjugated at 1/100000 dilution

Predicted band size: 29 kDa
Observed band size: 54 kDa
why is the actual band size different from the predicted?

Blocking and dilution buffer: 5% NFDM/TBST.

Exposure time: 3 minutes.
Immunocytochemistry/ Immunofluorescence - Anti-XBP1 antibody [143F] (ab241571)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Huh7 (human hepatocellular carcinoma epithelial cell) cells labeling XBP1 with ab241571 at 11.66 µg/ml, followed by ab150077 AlexaFluor^®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution (green). Confocal image showing increased signal staining in Huh7 cells treated with thapsigargin (300 nM) for 18 hours. Tubulin is detected with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) at 1/200 dilution (red). The nuclear counter stain is DAPI (blue).

Secondary antibody only control: Used PBS instead of primary antibody, followed by ab150077 AlexaFluor^®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-XBP1 antibody [143F] (ab241571)

Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling XBP1 with ab241571 at 1.166 µg/ml, followed by Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879) ready to use. Nuclear staining on the human tonsil is observed. Counter stained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879) ready to use.

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-XBP1 antibody [143F] (ab241571)

Immunohistochemical analysis of paraffin-embedded human non-Hodgkin's lymphoma tissue labeling XBP1 with ab241571 at 1.166 µg/ml, followed by Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879) ready to use. Nuclear staining on the non-Hodgkin's lymphoma is observed. Counter stained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879) ready to use.

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Anti-XBP1 antibody [143F] (ab241571)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com