Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-NELF-B antibody [EPR11200] (ab167401)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR11200] to NELF-B
  • Suitable for: WB, ICC/IF, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-NELF-B antibody [EPR11200]
    See all NELF-B primary antibodies

  • Description

    Rabbit monoclonal [EPR11200] to NELF-B

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment within Human NELF-B. The exact sequence is proprietary.
    Database link: Q8WX92

  • Positive control

    • T47D, HeLa, BxPC-3 and HepG2 whole cell lysate (ab7900); Human colon and Human uterus tissues; BxPC-3 cells.

  • General notes

     

     

    Previously labelled as COBRA1. 

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR11200

  • Isotype

    IgG

  • Research areas

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NELF-B antibody [EPR11200] (ab167401)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NELF-B antibody [EPR11200] (ab167401)

    Formalin-fixed, paraffin-embedded human kidney tissue stained for NELF-B with ab167401 (1/100 dilution) in immunohistochemical analysis.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NELF-B antibody [EPR11200] (ab167401)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NELF-B antibody [EPR11200] (ab167401)

    Formalin-fixed, paraffin-embedded human pancreas tissue stained for NELF-B with ab167401 (1/100 dilution) in immunohistochemical analysis.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-NELF-B antibody [EPR11200] (ab167401)
    Immunocytochemistry/ Immunofluorescence - Anti-NELF-B antibody [EPR11200] (ab167401)

    Immunofluorescent analysis of BxPC-3 cells labeling NELF-B with ab167401 at 1/100 dilution.

  • Western blot - Anti-NELF-B antibody [EPR11200] (ab167401)
    Western blot - Anti-NELF-B antibody [EPR11200] (ab167401)
    All lanes : Anti-NELF-B antibody [EPR11200] (ab167401) at 1/1000 dilution

    Lane 1 : T47D cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : BxPC-3 cell lysate
    Lane 4 : HepG2 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 66 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NELF-B antibody [EPR11200] (ab167401)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NELF-B antibody [EPR11200] (ab167401)

    Formalin-fixed, paraffin-embedded human colon tissue stained for NELF-B with ab167401 (1/100 dilution) in immunohistochemical analysis.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NELF-B antibody [EPR11200] (ab167401)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NELF-B antibody [EPR11200] (ab167401)

    Formalin-fixed, paraffin-embedded human uterus tissue stained for NELF-B with ab167401 (1/100 dilution) in immunohistochemical analysis.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Anti-NELF-B antibody [EPR11200] (ab167401)
    Anti-NELF-B antibody [EPR11200] (ab167401)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-NELF-B antibody [EPR11200] (ab167401)

© 2021 Astana Biomed Group LLP. All rights reserved.