Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PDGFR beta antibody [42G12] (ab69506)

Key features and details

  • Mouse monoclonal [42G12] to PDGFR beta
  • Suitable for: IHC-P, WB, Flow Cyt
  • Knockout validated
  • Reacts with: Mouse, Human
  • Isotype: IgG1

Overview

  • Product name

    Anti-PDGFR beta antibody [42G12]
    See all PDGFR beta primary antibodies

  • Description

    Mouse monoclonal [42G12] to PDGFR beta

  • Host species

    Mouse

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Mouse
    Human
    IHC-P
    Human
    WB
    Mouse
    Human
    See all applications and species data

  • Immunogen

    Recombinant full length protein corresponding to Human PDGFR beta.

  • Positive control

    • IHC-P: Human spleen and placenta tissues; Mouse heart muscle lysate. WB: Wild-type SH-SY5Y cell lysate. Flow Cyt: NIH 3T3 cells.

  • General notes

    This product was changed from ascites to tissue culture supernatant on 22nd May 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDGFR beta antibody [42G12] (ab69506)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDGFR beta antibody [42G12] (ab69506)

    Immunohistochemistry analysis of human spleen tissue labeling PDGFR beta [42G12] with ab69506 at 20µg/ml.

    This image was generated using the ascites version of the product.

  • Western blot - Anti-PDGFR beta antibody [42G12] (ab69506)
    Western blot - Anti-PDGFR beta antibody [42G12] (ab69506)
    All lanes : Anti-PDGFR beta antibody [42G12] (ab69506) at 1/1000 dilution

    Lane 1 : Wild-type SH-SY5Y cell lysate
    Lane 2 : PDGFRB knockout SH-SY5Y cell lysate
    Lane 3 : Human Skeletal Muscle tissue lysate
    Lane 4 : HeLa cell lysate

    Lysates/proteins at 30 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 124 kDa
    Observed band size: 170 kDa
    why is the actual band size different from the predicted?



    Lanes 1 - 4: Merged signal (red and green). Green - ab69506 observed at 170 kDa. Red - loading control ab52866 (Rabbit anti-alpha Tubulin antibody [EP1332Y]) observed at 55 kDa.

    ab69506 was shown to react with PDGFR beta in wild-type SH-SY5Y cells in Western blot with loss of signal observed in PDGFRB knockout cell line ab273749 (knockout cell lysate ab275523). Wild-type SH-SY5Y and PDGFRB knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween®) before incubation with ab69506 and ab52866 (Rabbit anti-alpha Tubulin antibody [EP1332Y]) overnight at 4 °C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed (ab216777) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDGFR beta antibody [42G12] (ab69506)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDGFR beta antibody [42G12] (ab69506)

    Immunohistochemistry analysis of human placenta tissue labeling PDGFR beta [42G12] with ab69506 at at 20µg/ml.

    This image was generated using the ascites version of the product.

  • Flow Cytometry - Anti-PDGFR beta antibody [42G12] (ab69506)
    Flow Cytometry - Anti-PDGFR beta antibody [42G12] (ab69506)

    Overlay histogram showing NIH 3T3 cells stained with ab69506 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab69506, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

    This image was generated using the ascites version of the product.

  • Western blot - Anti-PDGFR beta antibody [42G12] (ab69506)
    Western blot - Anti-PDGFR beta antibody [42G12] (ab69506)
    All lanes : Anti-PDGFR beta antibody [42G12] (ab69506) at 1 µg/ml

    Lane 1 : Molecular Weight Marker
    Lane 2 : Mouse Heart Muscle lysate

    Developed using the ECL technique.

    Predicted band size: 124 kDa
    Observed band size: 124 kDa



    Note: extra bands are secondary reactivity

    This image was generated using the ascites version of the product.

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