Anti-PDGFR beta (phospho Y1021) antibody [EPR2476] (ab134048)
![Anti-PDGFR beta (phospho Y1021) antibody [EPR2476] (ab134048)](https://www.abcam.com/ps/products/134/ab134048/Images/PDGF-Receptor-beta-Primary-antibodies-ab134048-1.jpg "Anti-PDGFR beta (phospho Y1021) antibody [EPR2476] (ab134048)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2476] to PDGFR beta (phospho Y1021)
- Suitable for: WB, Flow Cyt, Dot blot
- Reacts with: Mouse
Overview
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Product name
Anti-PDGFR beta (phospho Y1021) antibody [EPR2476]
See all PDGFR beta primary antibodies -
Description
Rabbit monoclonal [EPR2476] to PDGFR beta (phospho Y1021) -
Host species
Rabbit -
Specificity
Only detects PDGFR beta phosphorylated at tyrosine 1021.
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Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt MouseWB Mouse
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Immunogen
corresponding to Human PDGFR beta.
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Positive control
- NIH/3T3 lysates treated with Pervanadate
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General notes
Human, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR2476 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-PDGFR beta (phospho Y1021) antibody [EPR2476] (ab134048)All lanes : Anti-PDGFR beta (phospho Y1021) antibody [EPR2476] (ab134048) at 1/50000 dilution
Lane 1 : NIH/3T3 untreated
Lane 2 : NIH/3T3 treated with Pervanadate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 190 kDa
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![Flow Cytometry - Anti-PDGFR beta (phospho Y1021) antibody [EPR2476] (ab134048)](https://www.abcam.com/ps/products/134/ab134048/Images/ab134048-414376-AntiPDGFR-beta-phospho-Y1021-antibody.jpg)
Flow Cytometry - Anti-PDGFR beta (phospho Y1021) antibody [EPR2476] (ab134048)Flow cytoremtic analysis of permeabilized NIH/3T3 cells, untreated (green) or pervanadate-treated (red) using anti-PDGF Receptor beta phospho RabMab and pervanadate-treated NIH/3T3 cells using the same antibody preincubated with phospho-PDGF Receptor beta peptide (blue) or non-phospho-PDGF Receptor beta peptide (orange)
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![Western blot - Anti-PDGFR beta (phospho Y1021) antibody [EPR2476] (ab134048)](https://www.abcam.com/ps/products/134/ab134048/Images/ab134048-307294-ab134048WB1.jpg)
Western blot - Anti-PDGFR beta (phospho Y1021) antibody [EPR2476] (ab134048)All lanes : Anti-PDGFR beta (phospho Y1021) antibody [EPR2476] (ab134048) at 1/5000 dilution (Purified)
Lane 1 : NIH/3T3 (mouse embryonic fibroblast) whole cell lysate
Lane 2 : NIH/3T3 treated with 1mM pervanadate for 30 minutes whole cell lysate
Lane 3 : NIH/3T3 treated with 1mM pervanadate for 30 minutes whole cell lysate. Then the membrane was incubated with alkaline phosphatase
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 190 kDaBlocking/Diluting Buffer and concentration 5% NFDM/TBST
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![Dot Blot - Anti-PDGFR beta (phospho Y1021) antibody [EPR2476] (ab134048)](https://www.abcam.com/ps/products/134/ab134048/Images/ab134048-307293-ab134048Dot1.jpg)
Dot Blot - Anti-PDGFR beta (phospho Y1021) antibody [EPR2476] (ab134048)Dot blot analysis of PDGFR beta (phospho Y1021) phospho peptide (Lane 1) and PDGFR beta non-phospho peptide (Lane 2) labeling PDGFR beta (phospho Y1021) phospho peptide with ab134048 at a dilution of 1/1000 dilution (0.54ug/ml). A Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) was used as the secondary antibody at a dilution of 1/20,000 dilution.
Blocking buffer: 5% NFDM/TBST. Dilution buffer: 5% NFDM /TBST .
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![Anti-PDGFR beta (phospho Y1021) antibody [EPR2476] (ab134048)](https://www.abcam.com/ps/products/134/ab134048/Images/ab134048-5-benefits-of-recombinant-antibodies.png)
Anti-PDGFR beta (phospho Y1021) antibody [EPR2476] (ab134048)
