Antibodies, Proteins, Kits and Reagents for Life Science

278 083 ₸ Product size

100 µl 278 083 ₸

Order now and get it on Tuesday March 02, 2021

Anti-HRPT2/Parafibromin antibody [EPR19927] (ab223840)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR19927] to HRPT2/Parafibromin
Suitable for: WB, IHC-P, Flow Cyt
Reacts with: Mouse, Rat, Human

Product name

Anti-HRPT2/Parafibromin antibody [EPR19927]
See all HRPT2/Parafibromin primary antibodies
Description

Rabbit monoclonal [EPR19927] to HRPT2/Parafibromin
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
IHC-P	Mouse Rat Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: HeLa, Caco-2 and HepG2 whole cell lysates; Human fetal kidney lysate. IHC-P: Human kidney and parathyroid carcinoma tissues; Mouse and rat brain tissue. Flow Cytometry: HeLa cells.
General notes
This product was previously labelled as HRPT2

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine)
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR19927
Isotype

IgG
Research areas

Western blot - Anti-HRPT2/Parafibromin antibody [EPR19927] (ab223840)

All lanes : Anti-HRPT2/Parafibromin antibody [EPR19927] (ab223840) at 1/1000 dilution

Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg
Lane 2 : Caco-2 (human colorectal adenocarcinoma cell line) whole cell lysate at 20 µg
Lane 3 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate at 20 µg
Lane 4 : Human fetal kidney lysate at 10 µg

Secondary
Lanes 1-3 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Lane 4 : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

Developed using the ECL technique.

Predicted band size: 61 kDa
Observed band size: 60 kDa
why is the actual band size different from the predicted?

Blocking/Dilution buffer: 5% NFDM/TBST.

Exposure time : Lanes 1-3: 30 seconds Lane 4: 3 minutes.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HRPT2/Parafibromin antibody [EPR19927] (ab223840)

Immunohistochemical analysis of paraffin-embedded human kidney (A) and human clear cell renal cell carcinoma (B) labeling HRPT2/Parafibromin with ab223840 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear staining of human kidney epithelium; human clear cell renal cell carcinoma is negative for HRPT2/Parafibromin (PMID: 23361235). Counter stained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Flow Cytometry - Anti-HRPT2/Parafibromin antibody [EPR19927] (ab223840)

Flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cell line labeling HRPT2/Parafibromin with ab223840 at 1/500 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HRPT2/Parafibromin antibody [EPR19927] (ab223840)

Immunohistochemical analysis of paraffin-embedded human parathyroid carcinoma case 1 (A) and human parathyroid carcinoma case 2 (B) labeling HRPT2/Parafibromin with ab223840 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear staining observed in one parathyroid carcinoma case, while another case was found to be negative for the target (PMID: 16728578). Counter stained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HRPT2/Parafibromin antibody [EPR19927] (ab223840)

Immunohistochemical analysis of paraffin-embedded mouse brain tissue labeling HRPT2/Parafibromin with ab223840 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear staining in neurons in murine neurons is observed. Counter stained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HRPT2/Parafibromin antibody [EPR19927] (ab223840)

Immunohistochemical analysis of paraffin-embedded rat brain tissue labeling HRPT2/Parafibromin with ab223840 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear staining in neurons in rat brain is observed. Counter stained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Anti-HRPT2/Parafibromin antibody [EPR19927] (ab223840)

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