Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-HRPT2/Parafibromin antibody [EPR19927] (ab223840)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19927] to HRPT2/Parafibromin
  • Suitable for: WB, IHC-P, Flow Cyt
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-HRPT2/Parafibromin antibody [EPR19927]
    See all HRPT2/Parafibromin primary antibodies

  • Description

    Rabbit monoclonal [EPR19927] to HRPT2/Parafibromin

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IHC-P
    Mouse
    Rat
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa, Caco-2 and HepG2 whole cell lysates; Human fetal kidney lysate. IHC-P: Human kidney and parathyroid carcinoma tissues; Mouse and rat brain tissue. Flow Cytometry: HeLa cells.

  • General notes

     This product was previously labelled as HRPT2

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine)
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR19927

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-HRPT2/Parafibromin antibody [EPR19927] (ab223840)
    Western blot - Anti-HRPT2/Parafibromin antibody [EPR19927] (ab223840)
    All lanes : Anti-HRPT2/Parafibromin antibody [EPR19927] (ab223840) at 1/1000 dilution

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg
    Lane 2 : Caco-2 (human colorectal adenocarcinoma cell line) whole cell lysate at 20 µg
    Lane 3 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate at 20 µg
    Lane 4 : Human fetal kidney lysate at 10 µg

    Secondary
    Lanes 1-3 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
    Lane 4 : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

    Developed using the ECL technique.

    Predicted band size: 61 kDa
    Observed band size: 60 kDa
    why is the actual band size different from the predicted?



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time : Lanes 1-3: 30 seconds Lane 4: 3 minutes.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HRPT2/Parafibromin antibody [EPR19927] (ab223840)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HRPT2/Parafibromin antibody [EPR19927] (ab223840)

    Immunohistochemical analysis of paraffin-embedded human kidney (A) and human clear cell renal cell carcinoma (B) labeling HRPT2/Parafibromin with ab223840 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear staining of human kidney epithelium; human clear cell renal cell carcinoma is negative for HRPT2/Parafibromin (PMID: 23361235). Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Flow Cytometry - Anti-HRPT2/Parafibromin antibody [EPR19927] (ab223840)
    Flow Cytometry - Anti-HRPT2/Parafibromin antibody [EPR19927] (ab223840)

    Flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cell line labeling HRPT2/Parafibromin with ab223840 at 1/500 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HRPT2/Parafibromin antibody [EPR19927] (ab223840)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HRPT2/Parafibromin antibody [EPR19927] (ab223840)

    Immunohistochemical analysis of paraffin-embedded human parathyroid carcinoma case 1 (A) and human parathyroid carcinoma case 2 (B) labeling HRPT2/Parafibromin with ab223840 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear staining observed in one parathyroid carcinoma case, while another case was found to be negative for the target (PMID: 16728578). Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HRPT2/Parafibromin antibody [EPR19927] (ab223840)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HRPT2/Parafibromin antibody [EPR19927] (ab223840)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue labeling HRPT2/Parafibromin with ab223840 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear staining in neurons in murine neurons is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HRPT2/Parafibromin antibody [EPR19927] (ab223840)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HRPT2/Parafibromin antibody [EPR19927] (ab223840)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue labeling HRPT2/Parafibromin with ab223840 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear staining in neurons in rat brain is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Anti-HRPT2/Parafibromin antibody [EPR19927] (ab223840)
    Anti-HRPT2/Parafibromin antibody [EPR19927] (ab223840)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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