Anti-HRPT2/Parafibromin antibody (ab70533)
Key features and details
- Rabbit polyclonal to HRPT2/Parafibromin
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-HRPT2/Parafibromin antibody
See all HRPT2/Parafibromin primary antibodies -
Description
Rabbit polyclonal to HRPT2/Parafibromin -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
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Immunogen
Synthetic peptide corresponding to Human HRPT2/Parafibromin.
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Positive control
- Whole cell lysate from control 293T cells or 293T cells transfected with a HRPT2/Parafibromin expression construct.
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General notes
This product was previously labelled as HRPT2
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab70533 was affinity purified using an epitope specific to HRPT2/Parafibromin immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-HRPT2/Parafibromin antibody (ab70533)All lanes : Anti-HRPT2/Parafibromin antibody (ab70533) at 0.1 µg/ml
Lane 1 : Whole cell lysate from 293T cells at 10 µg
Lane 2 : Whole cell lysate from 293T cells at 20 µg
Lane 3 : Whole cell lysate from 293T cells transfected
with a HRPT2HRPT2/Parafibromin expression construct at 10 µg
Developed using the ECL technique.
Predicted band size: 61 kDa
Observed band size: 64 kDa why is the actual band size different from the predicted?
Exposure time: 5 minutes
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Immunoprecipitation - Anti-HRPT2/Parafibromin antibody (ab70533)Detection of Human HRPT2/Parafibromin (approximately 64 kDa) by Immunoprecipitation in whole cell lysate from 293T cells (10 mg for IP) in the presence or absence of blocking peptide, using ab70533 (BL648) at 3 µg/10mg lysate for IP and at 0.2 µg/ml for subsequent WB detection.
