Antibodies, Proteins, Kits and Reagents for Life Science

Key features and details

Mouse monoclonal [OTI9C6] to CD32A
Suitable for: WB, IHC-P, ICC/IF, IP
Reacts with: Human, African green monkey
Isotype: IgG1

Product name

Anti-CD32A antibody [OTI9C6]
See all CD32A primary antibodies
Description

Mouse monoclonal [OTI9C6] to CD32A
Host species

Mouse

Tested Applications & Species

Application	Species
ICC/IF	African green monkey
IHC-P	Human
WB	Human Recombinant fragment

See all applications and species data

Immunogen

Recombinant full length protein corresponding to Human CD32A.
Positive control
- HEK293T cell lysate transfected with pCMV6-ENTRY CD32; Human Breast, Colon, Kidney, Lung, Ovary, Ovary adenocarcinoma, Pancreas, Thyroid, Thyroid carcinoma, Endometrium and Prostate tissues; COS7 cells transiently transfected with pCMV6-ENTRY CD32;
General notes

Dilute in PBS (pH7.3) before use. Avoid repeated freeze-thaws.

The clone number has been updated from 9C6 to OTI9C6, both clone numbers name the same antibody clone.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

Learn more here.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer

pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 48% PBS, 50% Glycerol, 1% BSA
Concentration information loading...
Purity

Protein G purified
Purification notes

Purified from TCS
Clonality

Monoclonal
Clone number

OTI9C6
Isotype

IgG1
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD32A antibody [OTI9C6] (ab124408)

ab124408, at 1/50 dilution, staining CD32A in paraffin-embedded Human breast tissue by Immunohistochemistry.
Western blot - Anti-CD32A antibody [OTI9C6] (ab124408)

All lanes : Anti-CD32A antibody [OTI9C6] (ab124408) at 1/2000 dilution

Lane 1 : HEK293T cell lysate transfected with pCMV6-ENTRY control
Lane 2 : HEK293T cell lysate transfected with pCMV6-ENTRY CD32

Lysates/proteins at 5 µg per lane.

Predicted band size: 35 kDa

HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC205503)
Immunoprecipitation - Anti-CD32A antibody [OTI9C6] (ab124408)

Immunoprecipitation of CD32A. Lane 1: Negative control, no IgG. Lane2: 2µg of ab124408 and 20µl (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed with lysate and incubated overnight. For each experiment, 500µl of DDK tagged CD32 overexpression lysates (at 1:5 dilution with HEK293T lysate) was used. Predicted band size : 35 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD32A antibody [OTI9C6] (ab124408)

ab124408, at 1/50 dilution, staining CD32A in paraffin-embedded Human colon tissue by Immunohistochemistry.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD32A antibody [OTI9C6] (ab124408)

ab124408, at 1/50 dilution, staining CD32A in paraffin-embedded Human kidney tissue by Immunohistochemistry.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD32A antibody [OTI9C6] (ab124408)

ab124408, at 1/50 dilution, staining CD32A in paraffin-embedded Human lung tissue by Immunohistochemistry.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD32A antibody [OTI9C6] (ab124408)

ab124408, at 1/50 dilution, staining CD32A in paraffin-embedded Human ovary tissue by Immunohistochemistry.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD32A antibody [OTI9C6] (ab124408)

ab124408, at 1/50 dilution, staining CD32A in paraffin-embedded Human ovary adenocarcinoma tissue by Immunohistochemistry.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD32A antibody [OTI9C6] (ab124408)

ab124408, at 1/50 dilution, staining CD32A in paraffin-embedded Human pancreas tissue by Immunohistochemistry.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD32A antibody [OTI9C6] (ab124408)

ab124408, at 1/50 dilution, staining CD32A in paraffin-embedded Human thyroid tissue by Immunohistochemistry.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD32A antibody [OTI9C6] (ab124408)

ab124408, at 1/50 dilution, staining CD32A in paraffin-embedded Human thyroid carcinoma tissue by Immunohistochemistry.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD32A antibody [OTI9C6] (ab124408)

ab124408, at 1/50 dilution, staining CD32A in paraffin-embedded Human endometrium tissue by Immunohistochemistry.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD32A antibody [OTI9C6] (ab124408)

ab124408, at 1/50 dilution, staining CD32A in paraffin-embedded Human prostate tissue by Immunohistochemistry.
Immunocytochemistry/ Immunofluorescence - Anti-CD32A antibody [OTI9C6] (ab124408)

ab124408, at 1/100 dilution, staining CD32A in COS7 cells transiently transfected with pCMV6-ENTRY CD32 by Immunofluorescence.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com