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Immunology Innate Immunity Macrophage / Inflamm.

Anti-CD32B + CD32A + CD32C antibody [EPR6657(2)] (ab155972)

Price and availability

304 886 ₸

Availability

Order now and get it on Thursday March 04, 2021

Anti-CD32B + CD32A + CD32C antibody [EPR6657(2)] (ab155972)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6657(2)] to CD32B + CD32A + CD32C
  • Suitable for: WB, IHC-P
  • Reacts with: Human

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Overview

  • Product name

    Anti-CD32B + CD32A + CD32C antibody [EPR6657(2)]
  • Description

    Rabbit monoclonal [EPR6657(2)] to CD32B + CD32A + CD32C
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide within Human CD32A. The exact sequence is proprietary.
    (Peptide available as ab211774)

  • Positive control

    • WB: K562, U937, Daudi, human placenta and human platelet lysates. IHC-P: Human stomach and tonsil tissues.
  • General notes

    Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR6657(2)
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Innate Immunity
    • Macrophage / Inflamm.
    • Immunology
    • Adaptive Immunity
    • B Cells
    • CD
    • Immunology
    • Immunoglobulins
    • Receptors
    • Stem Cells
    • Hematopoietic Progenitors
    • Lymphoid
    • B Lymphocytic Lineage
    • Stem Cells
    • Hematopoietic Progenitors
    • Lymphoid
    • T Lymphocytic Lineage
    • Stem Cells
    • Hematopoietic Progenitors
    • Myeloid
    • Monocytic Lineage
    • Stem Cells
    • Hematopoietic Progenitors
    • Myeloid
    • Neutrophil Lineage
    • Stem Cells
    • Hematopoietic Progenitors
    • Myeloid
    • Mast Cell Lineage
    • Immunology
    • Innate Immunity
    • Fc Receptors

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD32B + CD32A + CD32C antibody [EPR6657(2)] (ab155972)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD32B + CD32A + CD32C antibody [EPR6657(2)] (ab155972)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human tonsil tissue sections labeling CD32A + CD32B + CD32C with purified ab155972 at 1/1000 dilution (0.98 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

  • Western blot - Anti-CD32B + CD32A + CD32C antibody [EPR6657(2)] (ab155972)
    Western blot - Anti-CD32B + CD32A + CD32C antibody [EPR6657(2)] (ab155972)
    Anti-CD32B + CD32A + CD32C antibody [EPR6657(2)] (ab155972) at 1/10000 dilution (Purified) + U-937 (Human histiocytic lymphoma monocyte) whole cell lysate at 15 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 35 kDa
    Observed band size: 37-42 kDa
    why is the actual band size different from the predicted?

  • Western blot - Anti-CD32B + CD32A + CD32C antibody [EPR6657(2)] (ab155972)
    Western blot - Anti-CD32B + CD32A + CD32C antibody [EPR6657(2)] (ab155972)
    All lanes : Anti-CD32B + CD32A + CD32C antibody [EPR6657(2)] (ab155972) at 1/1000 dilution (unpurified)

    Lane 1 : K562 cell lysate
    Lane 2 : U937 cell lysate
    Lane 3 : Daudi cell lysate
    Lane 4 : Human placenta lysate
    Lane 5 : Human platelet lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labeled goat anti-rabbit IgG at 1/2000 dilution

    Predicted band size: 35 kDa
    Observed band size: 37-42 kDa why is the actual band size different from the predicted?

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD32B + CD32A + CD32C antibody [EPR6657(2)] (ab155972)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD32B + CD32A + CD32C antibody [EPR6657(2)] (ab155972)

    Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human stomach tissue labeling CD32A + CD32B + CD32C with ab155972 (unpurified) at 1/250 dilution

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD32B + CD32A + CD32C antibody [EPR6657(2)] (ab155972)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD32B + CD32A + CD32C antibody [EPR6657(2)] (ab155972)

    ab155972 (unpurified) showing -ve staining in Human skeletal muscle.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD32B + CD32A + CD32C antibody [EPR6657(2)] (ab155972)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD32B + CD32A + CD32C antibody [EPR6657(2)] (ab155972)

    ab155972 (unpurified) showing +ve staining in Human placenta.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD32B + CD32A + CD32C antibody [EPR6657(2)] (ab155972)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD32B + CD32A + CD32C antibody [EPR6657(2)] (ab155972)

    Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human tonsil tissue labeling CD32A + CD32B + CD32C with ab155972 (unpurified) at 1/250 dilution

  • Anti-CD32B + CD32A + CD32C antibody [EPR6657(2)] (ab155972)
    Anti-CD32B + CD32A + CD32C antibody [EPR6657(2)] (ab155972)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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