Anti-CD32B + CD32A + CD32C antibody [EPR6657(2)] (ab155972)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6657(2)] to CD32B + CD32A + CD32C
- Suitable for: WB, IHC-P
- Reacts with: Human
Overview
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Product name
Anti-CD32B + CD32A + CD32C antibody [EPR6657(2)] -
Description
Rabbit monoclonal [EPR6657(2)] to CD32B + CD32A + CD32C -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human -
Immunogen
Synthetic peptide within Human CD32A. The exact sequence is proprietary.
(Peptide available asab211774) -
Positive control
- WB: K562, U937, Daudi, human placenta and human platelet lysates. IHC-P: Human stomach and tonsil tissues.
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General notes
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR6657(2) -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human tonsil tissue sections labeling CD32A + CD32B + CD32C with purified ab155972 at 1/1000 dilution (0.98 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
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Anti-CD32B + CD32A + CD32C antibody [EPR6657(2)] (ab155972) at 1/10000 dilution (Purified) + U-937 (Human histiocytic lymphoma monocyte) whole cell lysate at 15 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 35 kDa
Observed band size: 37-42 kDa why is the actual band size different from the predicted?
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All lanes : Anti-CD32B + CD32A + CD32C antibody [EPR6657(2)] (ab155972) at 1/1000 dilution (unpurified)
Lane 1 : K562 cell lysate
Lane 2 : U937 cell lysate
Lane 3 : Daudi cell lysate
Lane 4 : Human placenta lysate
Lane 5 : Human platelet lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labeled goat anti-rabbit IgG at 1/2000 dilution
Predicted band size: 35 kDa
Observed band size: 37-42 kDa why is the actual band size different from the predicted?
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Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human stomach tissue labeling CD32A + CD32B + CD32C with ab155972 (unpurified) at 1/250 dilution
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ab155972 (unpurified) showing -ve staining in Human skeletal muscle.
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ab155972 (unpurified) showing +ve staining in Human placenta.
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Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human tonsil tissue labeling CD32A + CD32B + CD32C with ab155972 (unpurified) at 1/250 dilution
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