Anti-Histone H4 antibody [RM212] (ab222763)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [RM212] to Histone H4
- Suitable for: ICC/IF, WB
- Reacts with: Human, Recombinant fragment
Overview
-
Product name
Anti-Histone H4 antibody [RM212]
See all Histone H4 primary antibodies -
Description
Rabbit monoclonal [RM212] to Histone H4 -
Host species
Rabbit -
Specificity
ab222763 reacts to the Histone H4 protein, independent of post-translational modifications. No cross reactivity with other histone proteins. -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB HumanRecombinant fragment -
Immunogen
Synthetic peptide corresponding to Human Histone H4 (C terminal).
Database link: P62805 -
Positive control
- WB: Recombinant Histone H4 protein; A-375, HEK-293, HeLa and SK-MEL-2 whole cell lysates. ICC/IF: HeLa cells.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 1% BSA, 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
-
Purity
Protein A purified -
Purification notes
ab222763 was purified from an animal origin–free culture supernatant. -
Clonality
Monoclonal -
Clone number
RM212 -
Isotype
IgG
Images
-
HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Histone H4 (red) using ab222763 at 2 µg/ml in ICC/IF. Actin filaments have been labeled with fluorescein phalloidin (green).
-
All lanes : Anti-Histone H4 antibody [RM212] (ab222763) at 0.2 µg/ml
Lane 1 : A-375 (human malignant melanoma cell line) whole cell lysate
Lane 2 : HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 4 : SK-MEL-2 whole cell lysate
Predicted band size: 11 kDa
-
All lanes : Anti-Histone H4 antibody [RM212] (ab222763) at 0.2 µg/ml
Lane 1 : Recombinant Histone H2A protein
Lane 2 : Recombinant Histone H2B protein
Lane 3 : Recombinant Histone H3.1 protein
Lane 4 : Recombinant Histone H3.3 protein
Lane 5 : Recombinant Histone H4 protein
Predicted band size: 11 kDaab222763 is specific to Histone H4.
-