Anti-5HT3B receptor antibody (ab39629)
Key features and details
- Rabbit polyclonal to 5HT3B receptor
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-5HT3B receptor antibody
See all 5HT3B receptor primary antibodies -
Description
Rabbit polyclonal to 5HT3B receptor -
Host species
Rabbit -
Specificity
Specific to 5HT3B - does not recognise 5HT3A receptor This antibody recognizes the intracellular region of the 5HT3B subunit. -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide, corresponding to amino acids 320-338 of Human 5HT3B receptor (large intracellular loop region)
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.1% Sodium azide -
Concentration information loading... -
Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-5HT3B receptor antibody (ab39629)Immunohistochemistry of human hippocampus stained with ab39269 diluted 1/500. Immunoreactivity with 5HT3B receptor can be seen in (i)CA1 field, (ii)CA2, (iii)CA3 and (iv)CA4/dentate gyrus.
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Western blot - Anti-5HT3B receptor antibody (ab39629)All lanes : Anti-5HT3B receptor antibody (ab39629) at 1/500 dilution
Lane 1 : Lysate from HEK 293 cells
Lane 2 : Lysate from HEK 293 cells transfected with 5HT3A receptor
Lane 3 : Lysate from HEK 293 cells transfected with 5HT3A receptor and 5HT3B receptor
Lane 4 : Human hippocampus lysate
Predicted band size: 50 kDa
Observed band size: 50 kDa
Pre-incubation of ab39629 with its immunizing peptide (at 10uM) results in a loss of immunoreactivity (lower panel). -
Immunocytochemistry/ Immunofluorescence - Anti-5HT3B receptor antibody (ab39629)HEK 293 cells transfected with 5HT3B receptor were stained with ab39629 diluted 1/500 and anti-rabbit secondary antibody conjugated to Alexa Fluor 488 diluted 1/1000(green). Nuclei are labelled with Hoescht 33258 (blue).
