Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Histone H4 (tri methyl K20) antibody [EPR17001(2)] - ChIP Grade (ab177190)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR17001(2)] to Histone H4 (tri methyl K20) - ChIP Grade
  • Suitable for: IHC-P, WB, Dot blot, ChIP
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Histone H4 (tri methyl K20) antibody [EPR17001(2)] - ChIP Grade
    See all Histone H4 primary antibodies

  • Description

    Rabbit monoclonal [EPR17001(2)] to Histone H4 (tri methyl K20) - ChIP Grade

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ChIP
    Human
    Dot
    Human
    IHC-P
    Mouse
    Rat
    Human
    WB
    Mouse
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa and NIH/3T3 cell lysates. IHC-P: Human, mouse and rat colon tissues. ICC/IF: HeLa cells. ChIP: Chromatin prepared from HeLa cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • ChIP - Anti-Histone H4 (tri methyl K20) antibody [EPR17001(2)] - ChIP Grade (ab177190)
    ChIP - Anti-Histone H4 (tri methyl K20) antibody [EPR17001(2)] - ChIP Grade (ab177190)

    Chromatin was prepared from HeLa cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 25µg of chromatin, 2µg of ab177190 (blue), and 20µl of Protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach for active loci and Sybr green approach for heterochromatic loci). Primers and probes are located in the first kb of the transcribed region.

  • Western blot - Anti-Histone H4 (tri methyl K20) antibody [EPR17001(2)] - ChIP Grade (ab177190)
    Western blot - Anti-Histone H4 (tri methyl K20) antibody [EPR17001(2)] - ChIP Grade (ab177190)
    All lanes : Anti-Histone H4 (tri methyl K20) antibody [EPR17001(2)] - ChIP Grade (ab177190) at 1/1000 dilution

    Lane 1 : HeLa (Human epithelial cells from cervix adenocarcinoma) cell lysate
    Lane 2 : NIH 3T3 (Mouse embyro fibroblast cells) cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 11 kDa
    Observed band size: 11 kDa


    Exposure time: 1 minute


    5% NFDM/TBST: Blocking and diluting buffer.

  • Dot Blot - Anti-Histone H4 (tri methyl K20) antibody [EPR17001(2)] - ChIP Grade (ab177190)
    Dot Blot - Anti-Histone H4 (tri methyl K20) antibody [EPR17001(2)] - ChIP Grade (ab177190)

    Dot blot analysis of Histone H4 (tri methyl K20) peptide(aa16-25) (Lane 1) and unmodified Histone H4 peptide (aa 16-25) (Lane 2) labeled using ab177190 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated secondary antibody at 1/1000 dilution.

    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H4 (tri methyl K20) antibody [EPR17001(2)] - ChIP Grade (ab177190)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H4 (tri methyl K20) antibody [EPR17001(2)] - ChIP Grade (ab177190)

    Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling Histone H4 (tri methyl K20) using ab177190 at 1/8000 dilution. A Goat Anti-Rabbit IgG H&L (HRP) (ab97051) was used as secondary at 1/500 dilution. Nucleus staining on Human colon was observed. Counterstained with Hematoxylin.
    Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H4 (tri methyl K20) antibody [EPR17001(2)] - ChIP Grade (ab177190)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H4 (tri methyl K20) antibody [EPR17001(2)] - ChIP Grade (ab177190)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue labeling Histone H4 (tri methyl K20) using ab177190 at 1/8000 dilution. A Goat Anti-Rabbit IgG H&L (HRP) (ab97051) was used as secondary at 1/500 dilution. Nucleus staining on mouse colon was observed. Counterstained with Hematoxylin.
    Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H4 (tri methyl K20) antibody [EPR17001(2)] - ChIP Grade (ab177190)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H4 (tri methyl K20) antibody [EPR17001(2)] - ChIP Grade (ab177190)

    Immunohistochemical analysis of paraffin-embedded rat colon tissue labeling Histone H4 (tri methyl K20) using ab177190 at 1/8000 dilution. A Goat Anti-Rabbit IgG H&L (HRP) (ab97051) was used as secondary at 1/500 dilution. Nucleus staining on rat colon was observed. Counterstained with Hematoxylin.
    Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Anti-Histone H4 (tri methyl K20) antibody [EPR17001(2)] - ChIP Grade (ab177190)
    Anti-Histone H4 (tri methyl K20) antibody [EPR17001(2)] - ChIP Grade (ab177190)

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