Anti-Histone H4 antibody [mAbcam 17036] - ChIP Grade – BSA and Azide free (ab174628)
![Anti-Histone H4 antibody [mAbcam 17036] - ChIP Grade – BSA and Azide free (ab174628)](https://www.abcam.com/ps/products/174/ab174628/Images/ab174628-284211-anti-histone-h4-antibody-mabcam-17036-chip-grade-chip.jpg "Anti-Histone H4 antibody [mAbcam 17036] - ChIP Grade – BSA and Azide free (ab174628)")
Key features and details
- Mouse monoclonal [mAbcam 17036] to Histone H4 - ChIP Grade – BSA and Azide free
- Suitable for: IHC-P, ChIP, WB
- Reacts with: Human
- Isotype: IgG2a
Overview
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Product name
Anti-Histone H4 antibody [mAbcam 17036] - ChIP Grade – BSA and Azide free
See all Histone H4 primary antibodies -
Description
Mouse monoclonal [mAbcam 17036] to Histone H4 - ChIP Grade – BSA and Azide free -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species ChIP Human
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Immunogen
Synthetic peptide corresponding to Human Histone H4 aa 50 to the C-terminus. Synthetic peptide conjugated to KLH derived from within residues 50 to the C-terminus of Human Histone H4.
Read Abcam's proprietary immunogen policy (Peptide available as ab13843.)
Database link: P62805 -
Positive control
- This antibody gave a positive signal in the following: Calf thymus histone preparation; HeLa histone preparation; Histone H4 recombinant protein; NIH3T3 Whole Cell Lysate; MEF1 Whole Cell Lysate; PC12 Whole Cell Lysate.
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General notes
This antibody clone is manufactured by Abcam.
This product is the PBS-only buffer version of ab17036.
If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
IgG fraction -
Clonality
Monoclonal -
Clone number
mAbcam 17036 -
Myeloma
Sp2/0-Ag14 -
Isotype
IgG2a -
Light chain type
kappa
Images
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ChIP - Anti-Histone H4 antibody [mAbcam 17036] - ChIP Grade – BSA and Azide free (ab174628)
This ChIP data was generated using the same antibody clone in a different buffer formulation (ab17036).
Chromatin was prepared from HeLa cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 25µg of chromatin, 5µg of ab17036 (blue), and 20µl of protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach for active and inactive loci, Sybr green approach for heterochromatic loci). Primers and probes are located in the first kb of the transcribed region.
