Anti-Histone H4 (mono methyl K77) antibody [EPR17921(2)] (ab177828)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR17921(2)] to Histone H4 (mono methyl K77)
- Suitable for: PepArr, WB
- Reacts with: Mouse, Human
Overview
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Product name
Anti-Histone H4 (mono methyl K77) antibody [EPR17921(2)]
See all Histone H4 primary antibodies -
Description
Rabbit monoclonal [EPR17921(2)] to Histone H4 (mono methyl K77) -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species PepArr HumanWB MouseHuman -
Immunogen
This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HeLa and NIH/3T3 cell lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR17921(2) -
Isotype
IgG -
Research areas
Images
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Anti-Histone H4 (mono methyl K77) antibody [EPR17921(2)] (ab177828) at 1/1000 dilution + HeLa (Human epithelial cells from cervix adenocarcinoma) cell lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 11 kDa
Observed band size: 11 kDa
Exposure time: 1 minuteBlocking/Dilution buffer: 5% BSA/TBST.
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Anti-Histone H4 (mono methyl K77) antibody [EPR17921(2)] (ab177828) at 1/1000 dilution + NIH/3T3 (Mouse embyro fibroblast cells) cell lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 11 kDa
Observed band size: 11 kDa
Exposure time: 1 minuteBlocking/Dilution buffer: 5% BSA/TBST.
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ab177828 was tested in Peptide Array against 501 different modified and unmodified histone peptides; each peptide is printed on the array at six concentrations (each in triplicate).
Circle area represents affinity between the antibody and a peptide: all antigen-containing peptides are displayed as red circles, all other peptides as blue circles. The affinity is calculated as area under curve when antibody binding values are plotted against the corresponding peptide concentration. Each circle area is normalized to the peptide with the strongest affinity.
The complete dataset, including full list of all peptides and information on the position of each peptide in the diagram, can be downloaded here. -