Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-C15orf41 antibody [EPR21169-28] (ab215190)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR21169-28] to C15orf41
  • Suitable for: WB, IP
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-C15orf41 antibody [EPR21169-28]
    See all C15orf41 primary antibodies

  • Description

    Rabbit monoclonal [EPR21169-28] to C15orf41

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Mouse
    Rat
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HEK-293T, RAW 264.7, PC-12, NIH/3T3 and HeLa whole cell lysate. Mouse spleen and kidney lysate. IP: HEK-293 whole cell lysate.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, PBS
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR21169-28

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-C15orf41 antibody [EPR21169-28] (ab215190)
    Western blot - Anti-C15orf41 antibody [EPR21169-28] (ab215190)
    All lanes : Anti-C15orf41 antibody [EPR21169-28] (ab215190) at 1/1000 dilution

    Lane 1 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with scrambled siRNA control whole cell lysate
    Lane 2 : C15orf41 siRNA knockdown HEK-293T whole cell lysate
    Lane 3 : HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 32 kDa
    Observed band size: 32 kDa


    Exposure time: 3 minutes


    siRNA control and C15orf41 knockdown lysates were provided by an anonymous collaborator.

    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-C15orf41 antibody [EPR21169-28] (ab215190)
    Western blot - Anti-C15orf41 antibody [EPR21169-28] (ab215190)
    All lanes : Anti-C15orf41 antibody [EPR21169-28] (ab215190) at 1/1000 dilution

    Lane 1 : RAW 264.7 (mouse abelson murine leukemia virus-induced tumor macrophage) whole cell lysate
    Lane 2 : PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate
    Lane 3 : NIH/3T3 (mouse embryonic fibroblast) whole cell lysate
    Lane 4 : Mouse kidney lysate
    Lane 5 : Mouse spleen lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 32 kDa
    Observed band size: 32 kDa


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunoprecipitation - Anti-C15orf41 antibody [EPR21169-28] (ab215190)
    Immunoprecipitation - Anti-C15orf41 antibody [EPR21169-28] (ab215190)

    C15orf41 was immunoprecipitated from 0.35 mg HEK-293 (human embryonic kidney epithelial cell) whole cell lysate with ab215190 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab215190 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used at 1/5000 dilution.

    Lane 1: HEK-293 whole cell lysate 10 µg (Input).
    Lane 2: ab215190 IP in HEK-293 whole cell lysate.
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab215190 in HEK-293 whole cell lysate.

    Blocking/Dilution buffer: 5% NFDM/TBST.
    Exposure time: 3 minutes.

  • Anti-C15orf41 antibody [EPR21169-28] (ab215190)
    Anti-C15orf41 antibody [EPR21169-28] (ab215190)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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