Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CD73 antibody [NT5E/2646] - BSA and Azide free (ab259284)

Key features and details

  • Mouse monoclonal [NT5E/2646] to CD73 - BSA and Azide free
  • Suitable for: ICC, WB, IHC-P, Protein Array
  • Reacts with: Human
  • Isotype: IgG1

Overview

  • Product name

    Anti-CD73 antibody [NT5E/2646] - BSA and Azide free
    See all CD73 primary antibodies

  • Description

    Mouse monoclonal [NT5E/2646] to CD73 - BSA and Azide free

  • Host species

    Mouse

  • Tested applications

    Suitable for: ICC, WB, IHC-P, Protein Arraymore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant full length protein corresponding to Human CD73.
    Database link: P21589

  • Positive control

    • IHC-P: Human colon carcinoma and tonsil tissue. ICC: U-87 MG cells. WB: A431 and PC3 cell lysate.

  • General notes

    ab259284 is a PBS only version of ab257312.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD73 antibody [NT5E/2646] - BSA and Azide free (ab259284)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD73 antibody [NT5E/2646] - BSA and Azide free (ab259284)

    Formalin-fixed, paraffin-embedded human tonsil tissue stained for CD73 using ab257312 at 2 µg/ml in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab257312).

  • Western blot - Anti-CD73 antibody [NT5E/2646] - BSA and Azide free (ab259284)
    Western blot - Anti-CD73 antibody [NT5E/2646] - BSA and Azide free (ab259284)
    All lanes : Anti-CD73 antibody [NT5E/2646] - BSA and Azide free (ab259284) at 1 µg/ml

    Lane 1 : A431 (Human epidermoid carcinoma cell line) whole cell lysate
    Lane 2 : PC3 (Human prostate adenocarcinoma cell line) whole cell lysate

    Predicted band size: 63 kDa



    Western blot analysis labeling CD73 with ab259284 at 1 ug/ml.

  • Immunocytochemistry/ Immunofluorescence - Anti-CD73 antibody [NT5E/2646] - BSA and Azide free (ab259284)
    Immunocytochemistry/ Immunofluorescence - Anti-CD73 antibody [NT5E/2646] - BSA and Azide free (ab259284)

    Immunofluorescence staining of U-87 MG (human glioblastoma-astrocytoma epithelial cell line) cells using ab257312 followed by a goat anti-Mouse IgG conjugated to CF488 (green). Membrane stained with Phalloidin (Red).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab257312).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD73 antibody [NT5E/2646] - BSA and Azide free (ab259284)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD73 antibody [NT5E/2646] - BSA and Azide free (ab259284)

    Formalin-fixed, paraffin-embedded human colon carcinoma tissuse stained for CD73 using ab257312 at 2 µg/ml in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab257312).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD73 antibody [NT5E/2646] - BSA and Azide free (ab259284)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD73 antibody [NT5E/2646] - BSA and Azide free (ab259284)

    Formalin-fixed, paraffin-embedded human colon carcinoma tissue stained for CD73 using ab257312 at 2 µg/ml in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab257312).

  • Protein Array - Anti-CD73 antibody [NT5E/2646] - BSA and Azide free (ab259284)
    Protein Array - Anti-CD73 antibody [NT5E/2646] - BSA and Azide free (ab259284)

    Analysis of Protein Array containing more than 19,000 full-length human proteins using ab257312.

    Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD’s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD’s) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab257312).

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