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Anti-Endothelial Cell antibody [RECA-1] - BSA and Azide free (ab264524)

Anti-Endothelial Cell antibody [RECA-1] - BSA and Azide free (ab264524)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Mouse monoclonal [RECA-1] to Endothelial Cell - BSA and Azide free
  • Suitable for: IHC-P, IHC-Fr, IHC-G, IHC-FrFl, ICC/IF
  • Reacts with: Rat
  • Isotype: IgG1

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Overview

  • Product name

    Anti-Endothelial Cell antibody [RECA-1] - BSA and Azide free
    See all Endothelial Cell primary antibodies
  • Description

    Mouse monoclonal [RECA-1] to Endothelial Cell - BSA and Azide free
  • Host species

    Mouse
  • Tested applications

    Suitable for: IHC-P, IHC-Fr, IHC-G, IHC-FrFl, ICC/IFmore details
  • Species reactivity

    Reacts with: Rat
  • Immunogen

    corresponding to Endothelial Cell.

  • Positive control

    • Rat brain FFPE section (IHC)
  • General notes

    Ab264524 is a PBS only version of ab9774.

    RECA-1 reacts with rat endothelial cell antigen and showed to be reactive with all vascular endothelium in three tested rat strains of different MHC-haplotype; Lewis (TR-11), BN (RT-1n) and OA (RT-1u). RECA-1 recognises a cell surface antigen. The antibody has been successfully applied in staining of viable endothelial cells in vitro, and of vascular endothelium in vivo. No reactivity of RECA-1 was seen with other cells e.g. fibroblasts, leukocytes and non endothelial stromal cells nor with other various tested species other than rat e.g. mouse, rabbit, sheep, goat and human. RECA-1 is a promising antibody for rat endothelial cell studies, and in particular for further defining nature and function of endothelial cell-specific antigens.

    This antibody clone is manufactured by Abcam. If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at 4°C (stable for up to 12 months). Upon delivery aliquot. Store at +4°C. Do Not Freeze.
  • Storage buffer

    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Monoclonal
  • Clone number

    RECA-1
  • Isotype

    IgG1
  • Research areas

    • Tags & Cell Markers
    • Cell Type Markers
    • Epi / Endo-thelial

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Endothelial Cell antibody [RECA-1] - BSA and Azide free (ab264524)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Endothelial Cell antibody [RECA-1] - BSA and Azide free (ab264524)

    IHC image of Endothelial Cell staining in Rat brain FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab9774, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, L-arginine and sodium azide (ab9774).

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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