Anti-CD73 antibody [4G6E3] (ab202122)
![Anti-CD73 antibody [4G6E3] (ab202122)](https://www.abcam.com/ps/products/202/ab202122/Images/ab202122-243400-anti-cd73-antibody-4g6e3-flow-cytometry.jpg "Anti-CD73 antibody [4G6E3] (ab202122)")
Key features and details
- Mouse monoclonal [4G6E3] to CD73
- Suitable for: Flow Cyt, IHC-P, WB
- Knockout validated
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-CD73 antibody [4G6E3]
See all CD73 primary antibodies -
Description
Mouse monoclonal [4G6E3] to CD73 -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanIHC-P HumanWB Human
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Immunogen
Recombinant fragment corresponding to Human CD73 aa 1-250. Expressed in E. Coli.
Database link: P21589 -
Positive control
- Flow Cytometry: A431 cells; WB: A375, and A431 cell lysates; IHC-P: Human endometrial cancer and Human esophageal cancer tissues.
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General notes
This product was changed from ascites to supernatant. Lot no’s high than GR287343-13 are from Tissue Culture Supernatant
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: 99% PBS -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purified from tissue culture supernatant. -
Clonality
Monoclonal -
Clone number
4G6E3 -
Isotype
IgG1 -
Research areas
Images
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Flow Cytometry - Anti-CD73 antibody [4G6E3] (ab202122)
Flow cytometric analysis of A431 cells using ab202122 at 1/200 (green) and negative control (red).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD73 antibody [4G6E3] (ab202122)](https://www.abcam.com/ps/products/202/ab202122/Images/ab202122-243397-anti-cd73-antibody-4g6e3-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD73 antibody [4G6E3] (ab202122)Immunohistochemical analysis of paraffin-embedded Human esophageal cancer tissue labeling CD73 with ab202122 at 1/200 with DAB staining.
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![Western blot - Anti-CD73 antibody [4G6E3] (ab202122)](https://www.abcam.com/ps/products/202/ab202122/Images/ab202122-353576-anticd73antibody4g6e3westernblotknockouta431nt5ea375humanlysates.jpg)
Western blot - Anti-CD73 antibody [4G6E3] (ab202122)All lanes : Anti-CD73 antibody [4G6E3] (ab202122) at 1/1000 dilution
Lane 1 : Wild-type A-431 whole cell lysate
Lane 2 : NT5E knockout A-431 whole cell lysate
Lane 3 : A375 whole cell lysate
Lysates/proteins at 20 µg per lane.Lanes 1 - 3: Merged signal (red and green). Green - ab202122 observed at 63 kDa. Red - loading control, ab181602, observed at 37 kDa.
ab202122 was shown to recognize NT5E in wild-type A-431 cells as signal was lost at the expected MW in NT5E knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and NT5E knockout samples were subjected to SDS-PAGE. Ab202122 and ab181602 (Rabbit anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed ab216772 and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed ab216777 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-CD73 antibody [4G6E3] (ab202122)](https://www.abcam.com/ps/products/202/ab202122/Images/ab202122-243394-anti-cd73-antibody-4g6e3-western-blot.jpg)
Western blot - Anti-CD73 antibody [4G6E3] (ab202122)Anti-CD73 antibody [4G6E3] (ab202122) at 1/500 dilution + A431 cell lysate
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![Western blot - Anti-CD73 antibody [4G6E3] (ab202122)](https://www.abcam.com/ps/products/202/ab202122/Images/ab202122-243395-anti-cd73-antibody-4g6e3-western-blot.jpg)
Western blot - Anti-CD73 antibody [4G6E3] (ab202122)Anti-CD73 antibody [4G6E3] (ab202122) at 1/500 dilution + Human CD73 (AA: 30-250) recombinant proteinExpected MW is 26.6 kDa
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD73 antibody [4G6E3] (ab202122)](https://www.abcam.com/ps/products/202/ab202122/Images/ab202122-243396-anti-cd73-antibody-4g6e3-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD73 antibody [4G6E3] (ab202122)Immunohistochemical analysis of paraffin-embedded Human endometrial cancer tissue labeling CD73 with ab202122 at 1/200 with DAB staining.
