Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CD73 antibody [4G6E3] (ab202122)

Key features and details

  • Mouse monoclonal [4G6E3] to CD73
  • Suitable for: Flow Cyt, IHC-P, WB
  • Knockout validated
  • Reacts with: Human
  • Isotype: IgG1

Overview

  • Product name

    Anti-CD73 antibody [4G6E3]
    See all CD73 primary antibodies

  • Description

    Mouse monoclonal [4G6E3] to CD73

  • Host species

    Mouse

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment corresponding to Human CD73 aa 1-250. Expressed in E. Coli.
    Database link: P21589

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • Flow Cytometry: A431 cells; WB: A375, and A431 cell lysates; IHC-P: Human endometrial cancer and Human esophageal cancer tissues.

  • General notes

    This product was changed from ascites to supernatant. Lot no’s high than GR287343-13 are from Tissue Culture Supernatant

Properties

Images

  • Flow Cytometry - Anti-CD73 antibody [4G6E3] (ab202122)
    Flow Cytometry - Anti-CD73 antibody [4G6E3] (ab202122)

    Flow cytometric analysis of A431 cells using ab202122 at 1/200 (green) and negative control (red).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD73 antibody [4G6E3] (ab202122)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD73 antibody [4G6E3] (ab202122)

    Immunohistochemical analysis of paraffin-embedded Human esophageal cancer tissue labeling CD73 with ab202122 at 1/200 with DAB staining.

  • Western blot - Anti-CD73 antibody [4G6E3] (ab202122)
    Western blot - Anti-CD73 antibody [4G6E3] (ab202122)
    All lanes : Anti-CD73 antibody [4G6E3] (ab202122) at 1/1000 dilution

    Lane 1 : Wild-type A-431 whole cell lysate
    Lane 2 : NT5E knockout A-431 whole cell lysate
    Lane 3 : A375 whole cell lysate

    Lysates/proteins at 20 µg per lane.


    Lanes 1 - 3: Merged signal (red and green). Green - ab202122 observed at 63 kDa. Red - loading control, ab181602, observed at 37 kDa.

    ab202122 was shown to recognize NT5E in wild-type A-431 cells as signal was lost at the expected MW in NT5E knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and NT5E knockout samples were subjected to SDS-PAGE. Ab202122 and ab181602 (Rabbit anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed ab216772 and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed ab216777 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-CD73 antibody [4G6E3] (ab202122)
    Western blot - Anti-CD73 antibody [4G6E3] (ab202122)
    Anti-CD73 antibody [4G6E3] (ab202122) at 1/500 dilution + A431 cell lysate
  • Western blot - Anti-CD73 antibody [4G6E3] (ab202122)
    Western blot - Anti-CD73 antibody [4G6E3] (ab202122)
    Anti-CD73 antibody [4G6E3] (ab202122) at 1/500 dilution + Human CD73 (AA: 30-250) recombinant protein


    Expected MW is 26.6 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD73 antibody [4G6E3] (ab202122)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD73 antibody [4G6E3] (ab202122)

    Immunohistochemical analysis of paraffin-embedded Human endometrial cancer tissue labeling CD73 with ab202122 at 1/200 with DAB staining.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-CD73 antibody [4G6E3] (ab202122)

© 2021 Astana Biomed Group LLP. All rights reserved.