PEG Virus Precipitation Kit (ab102538)
Key features and details
- Assay type: Direct
- Sample type: Cell culture media, Other biological fluids
- Sensitivity: 40 %
Overview
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Product name
PEG Virus Precipitation Kit -
Sample type
Other biological fluids, Cell culture media -
Assay type
Direct -
Sensitivity
> 40 % -
Range
40 % - 100 % -
Product overview
Abcam's PEG Virus Precipitation Kit provides an easy, convenient and time-saving method to concentrate viruses without ultra-centrifugation. The kit can be used for small lab samples or large scale virus preparation with high yield and high viral titer. The kit can be used to concentrate retroviruses, baculoviruses, lentiviruses, and phages etc. in cell culture medium or environmental samples. Viruses can be concentrated over 100 fold. An optimized Virus Re-suspension Solution is provided to maximize viral recovery by 40-100% depending on the virus type and sources. The whole process uses non-toxic reagents. The concentrated virus can be used for infection, viral DNA or RNA purification, etc.
Visit our FAQs page for tips and troubleshooting. -
Notes
Viruses are usually produced at a low titer. They often need to be concentrated for storage or further applications. A quick, easy and inexpensive method is desired to concentrate viruses and remove impurities.
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 50 tests PEG Solution 1 x 125ml Virus Re-suspension Solution 1 x 10ml -
Research areas
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Relevance
Virus is usually produced at a low titer. It often needs to be concentrated for storage or further applications. A quick, easy and inexpensive method is desired to concentrate virus and remove impurities. PEG (Polyethylene glycol) Virus Precipitation provides an easy, convenient and time-saving method to concentrate virus without ultra-centrifugation. -
Alternative names
- Polyethylene glycol virus
Images
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Functional Studies - PEG Virus Precipitation Kit (ab102538)
Concentration of baculovirus: Low titer baculovirus (10 ml) expressing human Granzyme B was precipitated following the kit protocol and the precipitate was suspended in 1 ml of Virus Re-suspension Solution. Both low titer and precipitated baculovirus was subsequently used to infect SF9 Insect cells. The activity of recombinant Granzyme B secreted into the culture medium by low titer and precipitated baculovirus infected insect cells was monitored using ab157403 - Granzyme B Activity Assay Kit (Fluorometric).