Rat TNFR1 ELISA Kit (ab231925)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 8.1 pg/ml
- Range: 15.6 pg/ml - 1000 pg/ml
- Sample type: Cit plasma, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Rat
Overview
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Product name
Rat TNFR1 ELISA Kit
See all Soluble TNF Receptor I kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Rat serum 5 2.5% Inter-assay Sample n Mean SD CV% Rat serum 3 7.6% -
Sample type
Serum, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
8.1 pg/ml -
Range
15.6 pg/ml - 1000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 101 95% - 104% Hep Plasma 101 97% - 109% EDTA Plasma 95 93% - 97% Cit plasma 98 96% - 100% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Rat -
Product overview
Rat TNFR1 ELISA Kit (ab231925) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of TNFR1 protein in cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Rat TNFR1 with 8.1 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
umor necrosis factor receptor superfamily member 1A (TNF RI) is a transmembrane protein with an extracellular domain that binds to TNF alpha. This extracellular domain can be proteolytically cleaved to make soluble TNF RI. The standard protein in this kit is soluble TNF RI and the capture and detector antibodies were raised against this region of TNF RI.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Rat TNFR1 Capture Antibody 1 x 600µl 10X Rat TNFR1 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 4BR 1 x 6ml Plate Seals 1 unit Rat TNFR1 Lyophilized Recombinant Protein 2 vials Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Relevance
TNF RI (CD120a) is a 55 kDa transmembrane glycoprotein that is expressed by virtually all nucleated mammalian cells. Among the numerous cells known to express TNF RI are hepatocytes, monocytes and neutrophils, cardiac muscle cells, endothelial cells, and CD34+ hematopoietic progenitors. Both TNF alpha and TNF beta bind to TNF RI. Soluble TNF alpha binds with a Kda in the range of 20-60 pM, while TNF beta binds with a Kda equal to 650 pM. TNF RI relative to TNF RII seems to be the more physiologically relevant receptor, whereas TNF R2 appears to play a direct role in only a limited number of TNF responses. Soluble TNF RI, which blocks TNF alpha activity, has been identified in both urine and blood (1-3 ng/mL). Serum levels of sTNF receptors increase dramatically in certain pathological situations. -
Cellular localization
Cytoplasmic -
Alternative names
- CD120a
- TBP1
- TNFR1
see all -
Database links
- Entrez Gene: 25625 Rat
- SwissProt: P22934 Rat
- Unigene: 11119 Rat
Images
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Other - Rat TNFR1 ELISA Kit (ab231925)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Rat TNFR1 Competitor standard curve comparisonStandard curve comparison between Rat TNFR1 SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows comparable sensitivity.
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Example of rat TNFR1 standard curve.The TNFR1 standard curve was prepared as described in Section 10.
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Interpolated concentrations of native TNFR1 in rat serum and plasma samples.The concentrations of TNFR1 were measured in duplicates, interpolated from the TNFR1 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 6.25%, plasma (citrate) 12.5%, plasma (EDTA) 12.5%, and plasma (heparin) 12.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean TNFR1 concentration was determined to be 8,972 pg/mL in serum, 4,723 pg/mL in plasma (citrate), 8468 pg/mL in plasma (EDTA) and 6,505 pg/mL in plasma (heparin).
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Interpolated concentrations of spiked TNFR1 in rat cell culture media samples.The concentrations of TNFR1 were measured in duplicates, interpolated from the TNFR1 standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
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Sandwich ELISA - Rat TNFR1 ELISA Kit (ab231925)To learn more about the advantages of recombinant antibodies see here.
