Recombinant human FGFR2 (mutated V564F) protein (ab204104)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 85% Densitometry
- Active: Yes
- Tags: GST tag N-Terminus
- Suitable for: SDS-PAGE, Functional Studies, WB
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Product name
Recombinant human FGFR2 (mutated V564F) protein
See all FGFR2 proteins and peptides -
Biological activity
The specific activity of ab204104 was determined to be 260 nmol/min/mg
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Purity
> 85 % Densitometry. -
Expression system
Baculovirus infected Sf9 cells -
Accession
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Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
RMKNTTKKPDFSSQPAVHKLTKRIPLRRQVSAESSSSMNSNTPLVRITTR LSSTADTPMLAGVSEYELPEDPKWEFPRDKLTLGKPLGEGCFGQVVMAEA VGIDKDKPKEAVTVAVKMLKDDATEKDLSDLVSEMEMMKMIGKHKNIINL LGACTQDGPLYVIFEYASKGNLREYLRARRPPGMEYSYDINRVPEEQMTF KDLVSCTYQLARGMEYLASQKCIHRDLAARNVLVTENNVMKIADFGLARD INNIDYYKKTTNGRLPVKWMAPEALFDRVYTHQSDVWSFGVLMWEIFTLG GSPYPGIPVEELFKLLKEGHRMDKPANCTNELYMMMRDCWHAVPSQRPTF KQLVEDLDRILTLTTNEEYLDLSQPLEQYSPSYPDTRSSCSSGDDSVFSP DPMPYEPCLPQYPHINGSVKT -
Predicted molecular weight
74 kDa including tags -
Amino acids
285 to 704 -
Modifications
mutated V564F -
Tags
GST tag N-Terminus -
Additional sequence information
BC039243.
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Preparation and Storage
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Stability and Storage
Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
pH: 7.50
Constituents: 0.79% Tris HCl, 0.87% Sodium chloride, 0.31% Glutathione, 0.003% EDTA, 0.004% DTT, 0.002% PMSF, 25% Glycerol (glycerin, glycerine)This product is an active protein and may elicit a biological response in vivo, handle with caution.
Images
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The specific activity of FGFR2 (ab204104) was determined to be 481 nmol/min/mg as per activity assay protocol and was equivalent to 280 nmol/min/mg as per radiometric assay
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SDS PAGE analysis of ab204104
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Kinase assay using ab204104 showing the specific activity to be 260 nmol/min/mg.
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SDS-PAGE analysis of ab204104.