Antibodies, Proteins, Kits and Reagents for Life Science

284 784 ₸ Product size

100 µl 284 784 ₸

Order now and get it on Tuesday March 09, 2021

Anti-CDC7 antibody [EPR20337] (ab229187)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR20337] to CDC7
Suitable for: WB, IHC-P, IP
Reacts with: Mouse, Rat, Human

Product name

Anti-CDC7 antibody [EPR20337]
See all CDC7 primary antibodies
Description

Rabbit monoclonal [EPR20337] to CDC7
Host species

Rabbit

Tested Applications & Species

Application	Species
IHC-P	Mouse Rat Human
IP	Human
WB	Human

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: HeLa, MCF7 and Jurkat whole cell lysate; WI-38 cells serum deprived, thenincubated with 10% FBS, whole cell lysate. IHC-P: Human breast cancer tissue; human, rat and mouse testis tissue. IP: HeLa whole cell lysate.
General notes
Previously labelled as Cdc7 Kinase.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR20337
Isotype

IgG
Research areas

Western blot - Anti-CDC7 antibody [EPR20337] (ab229187)

All lanes : Anti-CDC7 antibody [EPR20337] (ab229187) at 1/1000 dilution

Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate
Lane 2 : MCF7 (human breast adenocarcinoma epithelial cell), whole cell lysate
Lane 3 : Jurkat (human T cell leukemia T lymphocyte), whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
Lanes 1-2 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Lane 3 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

Developed using the ECL technique.

Predicted band size: 64 kDa
Observed band size: 64 kDa

Blocking/Dilution buffer: 5%NFDM/TBST.

Exposure times:

Lanes 1-2: 3 minutes

Lane 3: 32 seconds

Lane 3 was developed on a BIO-RAD^® ChemiDoc™ MP instrument.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDC7 antibody [EPR20337] (ab229187)

Immunohistochemical analysis of paraffin-embedded rat testis tissue labeling CDC7 with ab229187 at 1/500 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Nuclear staining in germ cells of rat testis is observed. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Immunoprecipitation - Anti-CDC7 antibody [EPR20337] (ab229187)

CDC7 was immunoprecipitated from 0.35 mg HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate with ab229187 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab229187 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

Lane 1: HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate 10 µg (Input).
Lane 2: ab229187 IP in HeLa whole cell lysate (+).
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab229187 in HeLa whole cell lysate (-).

Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 10 seconds.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDC7 antibody [EPR20337] (ab229187)

Immunohistochemical analysis of paraffin-embedded mouse testis tissue labeling CDC7 with ab229187 at 1/500 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Nuclear staining in germ cells of mouse testis (PMID: 9722556) is observed. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDC7 antibody [EPR20337] (ab229187)

Immunohistochemical analysis of paraffin-embedded human testis tissue labeling CDC7 with ab229187 at 1/500 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Nuclear staining in germ cells of human testis (PMID: 9250678) is observed. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDC7 antibody [EPR20337] (ab229187)

Immunohistochemical analysis of paraffin-embedded human normal breast tissue (panel A) and human breast cancer tissue (panel B) labeling CDC7 with ab229187 at 1/500 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Weak nuclear Cdc7 expression (arrow) found in human breast epithelial cells while strong nuclear Cdc7 expression found in human breast cancer cells (PMID: 19896697) is observed. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Western blot - Anti-CDC7 antibody [EPR20337] (ab229187)

All lanes : Anti-CDC7 antibody [EPR20337] (ab229187) at 1/1000 dilution

Lane 1 : WI-38 (human fetal lung fibroblast) serum deprived (0.1% FBS) for 72 hours, whole cell lysate
Lane 2 : WI-38 serum deprived (0.1% FBS) for 72 hours, then added 10% FBS for 18 hours, whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Developed using the ECL technique.

Predicted band size: 64 kDa
Observed band size: 64 kDa

Exposure time: 3 minutes

Blocking/Dilution buffer: 5% NFDM/TBST.

The blot was developed on a BIO-RAD^® ChemiDoc™ MP instrument (PMID: 19318489).
Anti-CDC7 antibody [EPR20337] (ab229187)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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