Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CDC7 antibody [EPR20337] (ab229187)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR20337] to CDC7
  • Suitable for: WB, IHC-P, IP
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-CDC7 antibody [EPR20337]
    See all CDC7 primary antibodies

  • Description

    Rabbit monoclonal [EPR20337] to CDC7

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Mouse
    Rat
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa, MCF7 and Jurkat whole cell lysate; WI-38 cells serum deprived, thenincubated with 10% FBS, whole cell lysate. IHC-P: Human breast cancer tissue; human, rat and mouse testis tissue. IP: HeLa whole cell lysate.

  • General notes

    Previously labelled as Cdc7 Kinase.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-CDC7 antibody [EPR20337] (ab229187)
    Western blot - Anti-CDC7 antibody [EPR20337] (ab229187)
    All lanes : Anti-CDC7 antibody [EPR20337] (ab229187) at 1/1000 dilution

    Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate
    Lane 2 : MCF7 (human breast adenocarcinoma epithelial cell), whole cell lysate
    Lane 3 : Jurkat (human T cell leukemia T lymphocyte), whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Lanes 1-2 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
    Lane 3 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

    Developed using the ECL technique.

    Predicted band size: 64 kDa
    Observed band size: 64 kDa



    Blocking/Dilution buffer: 5%NFDM/TBST.

    Exposure times:

    Lanes 1-2: 3 minutes

    Lane 3: 32 seconds

    Lane 3 was developed on a BIO-RAD® ChemiDoc™ MP instrument.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDC7 antibody [EPR20337] (ab229187)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDC7 antibody [EPR20337] (ab229187)

    Immunohistochemical analysis of paraffin-embedded rat testis tissue labeling CDC7 with ab229187 at 1/500 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Nuclear staining in germ cells of rat testis is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

  • Immunoprecipitation - Anti-CDC7 antibody [EPR20337] (ab229187)
    Immunoprecipitation - Anti-CDC7 antibody [EPR20337] (ab229187)

    CDC7 was immunoprecipitated from 0.35 mg HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate with ab229187 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab229187 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

    Lane 1: HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate 10 µg (Input).
    Lane 2: ab229187 IP in HeLa whole cell lysate (+).
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab229187 in HeLa whole cell lysate (-).

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.
    Exposure time: 10 seconds.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDC7 antibody [EPR20337] (ab229187)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDC7 antibody [EPR20337] (ab229187)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue labeling CDC7 with ab229187 at 1/500 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Nuclear staining in germ cells of mouse testis (PMID: 9722556) is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDC7 antibody [EPR20337] (ab229187)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDC7 antibody [EPR20337] (ab229187)

    Immunohistochemical analysis of paraffin-embedded human testis tissue labeling CDC7 with ab229187 at 1/500 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Nuclear staining in germ cells of human testis (PMID: 9250678) is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDC7 antibody [EPR20337] (ab229187)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDC7 antibody [EPR20337] (ab229187)

    Immunohistochemical analysis of paraffin-embedded human normal breast tissue (panel A) and human breast cancer tissue (panel B) labeling CDC7 with ab229187 at 1/500 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Weak nuclear Cdc7 expression (arrow) found in human breast epithelial cells while strong nuclear Cdc7 expression found in human breast cancer cells (PMID: 19896697) is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

  • Western blot - Anti-CDC7 antibody [EPR20337] (ab229187)
    Western blot - Anti-CDC7 antibody [EPR20337] (ab229187)
    All lanes : Anti-CDC7 antibody [EPR20337] (ab229187) at 1/1000 dilution

    Lane 1 : WI-38 (human fetal lung fibroblast) serum deprived (0.1% FBS) for 72 hours, whole cell lysate
    Lane 2 : WI-38 serum deprived (0.1% FBS) for 72 hours, then added 10% FBS for 18 hours, whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 64 kDa
    Observed band size: 64 kDa


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

    The blot was developed on a BIO-RAD® ChemiDoc™ MP instrument (PMID: 19318489).

  • Anti-CDC7 antibody [EPR20337] (ab229187)
    Anti-CDC7 antibody [EPR20337] (ab229187)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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