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Signal Transduction Signaling Pathway G Protein Signaling Small G Proteins Other

Anti-BTK (phospho Y551) antibody [EP267Y] (ab40770)

Price and availability

291 484 ₸

Availability

Order now and get it on Thursday March 04, 2021

Anti-BTK (phospho Y551) antibody [EP267Y] (ab40770)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP267Y] to BTK (phospho Y551)
  • Suitable for: ICC/IF, WB
  • Reacts with: Human

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Overview

  • Product name

    Anti-BTK (phospho Y551) antibody [EP267Y]
    See all BTK primary antibodies
  • Description

    Rabbit monoclonal [EP267Y] to BTK (phospho Y551)
  • Host species

    Rabbit
  • Specificity

    The antibody will detect BTK phosphorylation on Tyrosine 551.
  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide within Human BTK. The exact sequence is proprietary.

  • Positive control

    • WB: Sample: Ramos treated with Pervanadate whole cell lysates. ICC/IF: Ramos cells
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 50% Glycerol (glycerin, glycerine), 0.05% BSA, 49% PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EP267Y
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Protein Phosphorylation
    • Tyrosine Kinases
    • Other
    • Immunology
    • Innate Immunity
    • TLR Signaling

Images

  • Western blot - Anti-BTK (phospho Y551) antibody [EP267Y] (ab40770)
    Western blot - Anti-BTK (phospho Y551) antibody [EP267Y] (ab40770)
    All lanes : Anti-BTK (phospho Y551) antibody [EP267Y] (ab40770) at 1/5000 dilution

    Lane 1 : Ramos (human Burkitt's lymphoma) treated with Pervanadate whole cell lysates

    The treatment protocols were not recorded.
    Lane 2 : Untreated Ramos (human Burkitt's lymphoma) whole cell lysates

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/1000 dilution

    Predicted band size: 74 kDa
    Observed band size: 74 kDa


    Exposure time: 30 seconds


    Blocking/Diluting buffer 5% NFDM/TBST

  • Immunocytochemistry/ Immunofluorescence - Anti-BTK (phospho Y551) antibody [EP267Y] (ab40770)
    Immunocytochemistry/ Immunofluorescence - Anti-BTK (phospho Y551) antibody [EP267Y] (ab40770)

    Immunocytochemistry/ Immunofluorescence analysis of Ramos (human Burkitt's lymphoma B lymphocyte) treated with pervanadate (1mM 30min) cells labeling BTK with purified ab40770 at 1/500 dilution (2 µg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/mL) dilution. DAPI (blue) was used as nuclear counterstain. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL) was used as the secondary antibody only control.

  • Anti-BTK (phospho Y551) antibody [EP267Y] (ab40770)
    Anti-BTK (phospho Y551) antibody [EP267Y] (ab40770)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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