Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-BTK (phospho Y223) antibody [EP420Y] - BSA and Azide free (ab190919)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP420Y] to BTK (phospho Y223) - BSA and Azide free
  • Suitable for: IP, Dot blot, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-BTK (phospho Y223) antibody [EP420Y] - BSA and Azide free
    See all BTK primary antibodies

  • Description

    Rabbit monoclonal [EP420Y] to BTK (phospho Y223) - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
    (Peptide available as ab214589)

  • General notes

    Ab190919 is the carrier-free version of ab68217. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab190919 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Dot Blot - Anti-BTK (phospho Y223) antibody [EP420Y] - BSA and Azide free (ab190919)
    Dot Blot - Anti-BTK (phospho Y223) antibody [EP420Y] - BSA and Azide free (ab190919)

    Dot blot analysis of BTK (phospho Y223) phospho peptide (Lane 1) and BTK phospho peptide (Lane 2) labeling BTK (phospho Y223) with ab68217 at a dilution of 1/1000. A Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) was used as the secondary antibody at a dilution of 1/20,000. Blocking buffer: 5% NFDM/TBST. Dilution buffer: 5% NFDM /TBST.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab68217).

  • Immunoprecipitation - Anti-BTK (phospho Y223) antibody [EP420Y] - BSA and Azide free (ab190919)
    Immunoprecipitation - Anti-BTK (phospho Y223) antibody [EP420Y] - BSA and Azide free (ab190919)

    ab68217 (purified) at 1/50 immunoprecipitating BTK (phospho Y223) in Ramos cells (Lane 1) treated with 1 mM Pervanadate for 30 min. For western blotting, a HRP-conjugated anti-rabbit IgG (H+L) was used as the secondary antibody (1/1000).

    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab68217).

  • Anti-BTK (phospho Y223) antibody [EP420Y] - BSA and Azide free (ab190919)
    Anti-BTK (phospho Y223) antibody [EP420Y] - BSA and Azide free (ab190919)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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