Antibodies, Proteins, Kits and Reagents for Life Science

284 784 ₸ Product size

100 µg 284 784 ₸

Order now and get it on Tuesday March 09, 2021

Anti-EpCAM antibody [Cocktail EGP40/826/837/1110/1120] (ab216136)

Key features and details

Mouse monoclonal [Cocktail EGP40/826/837/1110/1120] to EpCAM
Suitable for: IHC-P, WB, Flow Cyt
Knockout validated
Reacts with: Human
Isotype: IgG1

Product name

Anti-EpCAM antibody [Cocktail EGP40/826/837/1110/1120]
See all EpCAM primary antibodies
Description

Mouse monoclonal [Cocktail EGP40/826/837/1110/1120] to EpCAM
Host species

Mouse
Specificity

This antibody recognizes extracellular as well as intracellular domains of the epithelial cellular adhesion molecule (Ep-CAM).

Tested Applications & Species

Application	Species
Flow Cyt	Human
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

Recombinant full length protein corresponding to Human EpCAM aa 1 to the C-terminus.
Database link: P16422

Run BLAST with Run BLAST with
Positive control
- IHC-P: Human breast carcinoma tissue. WB: A431 and MCF7 cell lysates and human lung tissue lysate Flow Cyt: MCF7 cells.
General notes

It is a cocktail of four highly specific monoclonal antibodies that recognize extracellular as well as intracellular domains of the epithelial cellular adhesion molecule (EpCAM).

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.05% BSA
Concentration information loading...
Purity

Protein G purified
Clonality

Monoclonal
Clone number

Cocktail EGP40/826/837/1110/1120
Isotype

IgG1
Light chain type

kappa
Research areas

Western blot - Anti-EpCAM antibody [Cocktail EGP40/826/837/1110/1120] (ab216136)

All lanes : Anti-EpCAM antibody [Cocktail EGP40/826/837/1110/1120] (ab216136) at 0.5 µg/ml

Lane 1 : Wild-type A431 cell lysate
Lane 2 : EPCAM knockout A431 cell lysate
Lane 3 : MCF7 cell lysate
Lane 4 : HeLa cell lysate

Lysates/proteins at 20 µg per lane.

Performed under reducing conditions.

Predicted band size: 35 kDa
Observed band size: 40 kDa
why is the actual band size different from the predicted?

Lanes 1 - 4: Merged signal (red and green). Green - ab216136 observed at 40 kDa. Red - loading control ab52866 (Rabbit anti-alpha Tubulin antibody [EP1332Y]) observed at 55kDa.

ab216136 was shown to react with EpCAM in wild-type A431 cells in western blot with loss of signal observed in EPCAM knockout sample. Wild-type and EPCAM knockout A431 cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween^®) before incubation with ab216136 and ab52866 (Rabbit anti-alpha Tubulin antibody [EP1332Y]) overnight at 4°C at 0.5 µg/ml and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Mouse IgG H&L (IRDye^® 800CW) preabsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye^® 680RD) preabsorbed (ab216777) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
Flow Cytometry - Anti-EpCAM antibody [Cocktail EGP40/826/837/1110/1120] (ab216136)

Overlay histogram showing MCF-7 (Human breast adenocarcinoma) cells stained with ab216136 (blue) or isotype control (red). The cells were fixed with paraformaldehyde and incubated with ab216136 at 1 ug/million cells.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EpCAM antibody [Cocktail EGP40/826/837/1110/1120] (ab216136)

Immunohistochemical analysis of paraffin embedded, formalin fixed human breast carcinoma tissue labeling EpCAM with ab216136 at 1 μg/mL.
Western blot - Anti-EpCAM antibody [Cocktail EGP40/826/837/1110/1120] (ab216136)

Anti-EpCAM antibody [Cocktail EGP40/826/837/1110/1120] (ab216136) + Human lung tissue lysate

Predicted band size: 35 kDa

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