Anti-EpCAM antibody [E144] (ab32392)
![Anti-EpCAM antibody [E144] (ab32392)](https://www.abcam.com/ps/products/32/ab32392/Images/ab32392-412981-anti-epcam-antibody-e144-western-blot-a431-wt-a431-epcam-knockout-mcf7-hela2.jpg "Anti-EpCAM antibody [E144] (ab32392)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [E144] to EpCAM
- Suitable for: WB
- Knockout validated
- Reacts with: Human
Overview
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Product name
Anti-EpCAM antibody [E144]
See all EpCAM primary antibodies -
Description
Rabbit monoclonal [E144] to EpCAM -
Host species
Rabbit -
Specificity
This antibody is specific for human EpCAM. -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide within Human EpCAM aa 250 to the C-terminus (C terminal). The exact sequence is proprietary.
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Positive control
- WB: MCF7 and A431 cell lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
E144 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-EpCAM antibody [E144] (ab32392)All lanes : Anti-EpCAM antibody [E144] (ab32392) at 1/2000 dilution
Lane 1 : Wild-type A431 cell lysate
Lane 2 : EPCAM knockout A431 cell lysate
Lane 3 : MCF7 cell lysate
Lane 4 : HeLa cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 39 kDa
Observed band size: 40 kDa why is the actual band size different from the predicted?Lanes 1 - 4: Merged signal (red and green). Green - ab32392 observed at 40 kDa. Red - loading control ab7291 (Mouse anti-Alpha Tubulin [DM1A] observed at 55kDa.
ab32392 was shown to react with EpCAM in wild-type A431 cells in western blot with loss of signal observed in EpCAM knockout sample. Wild-type and EpCAM knockout A431 cell lysates were subjected to SDS-PAGE. Membranes were blocked in fluorescent western blot (TBS-based) blocking solution before incubation with ab32392 and ab7291 (Mouse anti-Alpha Tubulin [DM1A] overnight at 4°C at a 1 in 2000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-EpCAM antibody [E144] (ab32392)](https://www.abcam.com/ps/products/32/ab32392/Images/ab32392-44691-anti-epcam-antibody-e144-western-blot.jpg)
Western blot - Anti-EpCAM antibody [E144] (ab32392)Anti-EpCAM antibody [E144] (ab32392) at 1/2500 dilution + A431 cell lysate
Predicted band size: 39 kDa
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![Anti-EpCAM antibody [E144] (ab32392)](https://www.abcam.com/ps/products/32/ab32392/Images/ab32392-9-benefits-of-recombinant-antibodies.png)
Anti-EpCAM antibody [E144] (ab32392)
