Anti-Rootletin antibody (ab121653)
Key features and details
- Rabbit polyclonal to Rootletin
- Suitable for: WB, IHC-P, ICC
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Rootletin antibody -
Description
Rabbit polyclonal to Rootletin -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICCmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human Rootletin aa 406-483.
Sequence:VKRLEKQNLEKDQVNKDLTEKLEALESLRLQEQAALETEDGEGLQQTLRD LAQAVLSDSESGVQLSGSERTADASNGS
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Positive control
- IHC-P: Human fallopian tube, bronchus, liver, cerebral cortex and testis tissue; ICC: U2OS cells; WB: SCLC-21H.
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General notes
Previously labelled as CROCC.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rootletin antibody (ab121653)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human bronchus tissue labelling Rootletin with ab121653 at 1/50 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunocytochemistry - Anti-Rootletin antibody (ab121653)Immunocytochemistry analysis of U-2 OS (human bone osteosarcoma epithelial cell line) cells labelling Rootletin with ab121653 at 2 µg/mL.
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Western blot - Anti-Rootletin antibody (ab121653)Anti-Rootletin antibody (ab121653) at 0.4 µg/ml + SCLC-21H (human small cell lung carcinoma cell line) cell lysate
Predicted band size: 229 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rootletin antibody (ab121653)ab121653, at 1/150 dilution, staining Rootletin in Human fallopian tube tissue by Immunohistochemistry using formalin-fixed, paraffin-embedded tissue.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rootletin antibody (ab121653)Immunohistochemical analysis of human testis tissue labeling Rootletin with ab121653 at 1/50 dilution. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rootletin antibody (ab121653)Immunohistochemical analysis of human liver tissue labeling Rootletin with ab121653 at 1/50 dilution.