Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-BubR1 antibody [EPR22544-48] (ab254326)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22544-48] to BubR1
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-BubR1 antibody [EPR22544-48]
    See all BubR1 primary antibodies

  • Description

    Rabbit monoclonal [EPR22544-48] to BubR1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Mouse
    Rat
    Human
    WB
    Mouse
    Rat
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa and Raji cell lysate; Mouse testis, thymus and rat testis tissue lysate. IHC-P: Human breast cancer and testis tissue; Mouse and rat testis tissue. ICC/IF: SK-OV-3 and HeLa cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-BubR1 antibody [EPR22544-48] (ab254326)
    Western blot - Anti-BubR1 antibody [EPR22544-48] (ab254326)
    All lanes : Anti-BubR1 antibody [EPR22544-48] (ab254326) at 1/1000 dilution

    Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate
    Lane 2 : Raji (human Burkitt's lymphoma B lymphocyte), whole cell lysate
    Lane 3 : Mouse testis tissue lysate
    Lane 4 : Mouse thymus tissue lysate
    Lane 5 : Rat testis tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 119 kDa



    Blocking/dilution buffer and concentration: 5% NFDM/TBST

    The molecular weight observed is consistent with what has been described in the literature (PMID: 26889953).

    Exposure time:

    Lanes 1-2: 70 seconds

    Lane 3: 48 seconds

    Lanes 4-5: 3 minutes 

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BubR1 antibody [EPR22544-48] (ab254326)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BubR1 antibody [EPR22544-48] (ab254326)

    Immunohistochemical analysis of paraffin-embedded rat testis tissue labeling BubR1 with ab254326 at 1/1000 dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on rat testis (PMID: 20411023). The section was incubated with ab254326 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). 

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BubR1 antibody [EPR22544-48] (ab254326)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BubR1 antibody [EPR22544-48] (ab254326)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue labeling BubR1 with ab254326 at 1/1000 dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on mouse testis (PMID: 20411023). The section was incubated with ab254326 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). 

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BubR1 antibody [EPR22544-48] (ab254326)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BubR1 antibody [EPR22544-48] (ab254326)

    Immunohistochemical analysis of paraffin-embedded human testis tissue labeling BubR1 with ab254326 at 1/1000 dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human testis (PMID: 20411023). The section was incubated with ab254326 for 30 mins at room temperature. Counterstained with hematoxylin. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). 

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BubR1 antibody [EPR22544-48] (ab254326)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BubR1 antibody [EPR22544-48] (ab254326)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue labeling BubR1 with ab254326 at 1/1000 dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining on human breast cancer is observed. The section was incubated with ab254326 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). 

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-BubR1 antibody [EPR22544-48] (ab254326)
    Immunocytochemistry/ Immunofluorescence - Anti-BubR1 antibody [EPR22544-48] (ab254326)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized SK-OV-3 (human ovarian cancer epithelial cell) cells labeling BubR1 with ab254326 at 1/50 dilution, followed by a AlexaFluor®488 Goat anti-Rabbit secondary (ab150077) at 1/1000 dilution (Green). Confocal image showing specific staining on centromeres of SK-OV-3 cell line in M phase. The nuclear counterstain is DAPI (Blue). Tubulin was stained using Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is an AlexaFluor®488 Goat anti-Rabbit secondary (ab150077) at 1/1000 dilution 

  • Immunocytochemistry/ Immunofluorescence - Anti-BubR1 antibody [EPR22544-48] (ab254326)
    Immunocytochemistry/ Immunofluorescence - Anti-BubR1 antibody [EPR22544-48] (ab254326)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human cervix adenocarcinoma epithelial cell) cells labeling BubR1 with ab254326 at 1/50 dilution, followed by a AlexaFluor®488 Goat anti-Rabbit secondary (ab150077) at 1/1000 dilution (Green).Confocal image showing cytoplasmic staining in HeLa cell line. The nuclear counterstain is DAPI (Blue). Tubulin was stained using Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) at 1/200 dilution.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is an AlexaFluor®488 Goat anti-Rabbit secondary (ab150077) at 1/1000 dilution 

  • Immunocytochemistry/ Immunofluorescence - Anti-BubR1 antibody [EPR22544-48] (ab254326)
    Immunocytochemistry/ Immunofluorescence - Anti-BubR1 antibody [EPR22544-48] (ab254326)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human cervix adenocarcinoma epithelial cell) cells labeling BubR1 with ab254326 at 1/50 dilution, followed by a AlexaFluor®488 Goat anti-Rabbit secondary (ab150077) at 1/1000 dilution (Green). Confocal image showing specific staining on centromeres of HeLa cell line in M phase. The nuclear counterstain is DAPI (Blue). Tubulin was stained using Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) at 1/200 dilution.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is an AlexaFluor®488 Goat anti-Rabbit secondary (ab150077) at 1/1000 dilution 

  • Anti-BubR1 antibody [EPR22544-48] (ab254326)
    Anti-BubR1 antibody [EPR22544-48] (ab254326)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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